Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F18%3A00493934" target="_blank" >RIV/61389030:_____/18:00493934 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/s12870-018-1337-y" target="_blank" >http://dx.doi.org/10.1186/s12870-018-1337-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12870-018-1337-y" target="_blank" >10.1186/s12870-018-1337-y</a>

Result language

angličtina

Original language name

Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

Original language description

To explore poorly understood differences between primary and subsequent somatic embryogenic lines of plants, we induced secondary (2ry) and tertiary (3ry) lines from cotyledonary somatic embryos (SEs) of two Douglas-fir genotypes: SD4 and TD17. The 2ry lines exhibited significantly higher embryogenic potential (SE yields) than the 1ry lines initiated from zygotic embryos (SD4, 2155 vs 477, TD17, 240 vs 29 g(-1) f.w.). Moreover, we observed similar differences in yield between 2ry and 3ry lines of SD4 (2400 vs 3921 g(-1) f.w.). To elucidate reasons for differences in embryogenic potential induced by repetitive somatic embryogenesis we then compared 2ry vs 1ry and 2ry vs 3ry lines at histo-cytological (using LC-MS/MS) and proteomic levels.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

<a href="/en/project/LTC17030" target="_blank" >LTC17030: Contribution of superresolution microscopy and image analysis to the study of plant in vitro cultures with the emphasis on somatic embryogenesis of conifers</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Plant Biology

ISSN

1471-2229

e-ISSN

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Volume of the periodical

18

Issue of the periodical within the volume

AUG 10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

24

Pages from-to

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UT code for WoS article

000441272500004

EID of the result in the Scopus database

2-s2.0-85051540656