Transcriptomic response of Arabidopsis thaliana roots to naproxen and praziquantel
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F18%3A00496183" target="_blank" >RIV/61389030:_____/18:00496183 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.ecoenv.2018.09.081" target="_blank" >http://dx.doi.org/10.1016/j.ecoenv.2018.09.081</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ecoenv.2018.09.081" target="_blank" >10.1016/j.ecoenv.2018.09.081</a>
Alternative languages
Result language
angličtina
Original language name
Transcriptomic response of Arabidopsis thaliana roots to naproxen and praziquantel
Original language description
Exposition to pharmaceutical compounds released to the environment is considered as a potential risk for various organisms. We exposed Arabidopsis thaliana plants to naproxen (NAP) and praziquantel (PZQ) in 5 mu M concentration for 2 days and recorded transcriptomic response in their roots with the aim to estimate ecotoxicity and to identify gene candidates potentially involved in metabolism of both compounds. Nonsteroidal anti-inflammatory drug NAP up-regulated 105 and down-regulated 29 genes (p-value <= 0.1, fold change >= 2), while anthelmintic PZQ up-regulated 389 and down-regulated 353 genes with more rigorous p-value <= 0.001 (fold change >= 2). High number of up-regulated genes coding for heat shock proteins and other genes involved in response to biotic and abiotic stresses as well as down-regulation of genes involved in processes such as cell proliferation, transcription and water transport indicates serious negative effect of PZQ. NAP up-regulated mostly genes involved in various biological processes and signal transduction and down-regulated mainly genes involved in signal transduction and electron transport or energy pathways. Further, two cytochrome P450s (demethylation) and one methyltransferase (methylation of carboxyl group) were identified as candidates for phase I and several glutathione- and glycosyltransferases (conjugation) for phase II of NAP metabolism. Cytochrome P450s, glutathione and glycosyltransferases seem to play role also in metabolism of PZQ. Up-regulation of several ABC and MATE transporters by NAP and PZQ indicated their role in transport of both compounds.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Ecotoxicology and Enviromental Safety
ISSN
0147-6513
e-ISSN
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Volume of the periodical
166
Issue of the periodical within the volume
DEC 30
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
301-310
UT code for WoS article
000448760800039
EID of the result in the Scopus database
2-s2.0-85054019204