Deciphering the phenolic acid reserves and antioxidant activity within the protocorm like bodies of Ansellia africana: A vulnerable medicinal orchid

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00506095" target="_blank" >RIV/61389030:_____/19:00506095 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/19:73597179

Result on the web

<a href="http://doi.org/10.1016/j.indcrop.2019.03.024" target="_blank" >http://doi.org/10.1016/j.indcrop.2019.03.024</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.indcrop.2019.03.024" target="_blank" >10.1016/j.indcrop.2019.03.024</a>

Result language

angličtina

Original language name

Deciphering the phenolic acid reserves and antioxidant activity within the protocorm like bodies of Ansellia africana: A vulnerable medicinal orchid

Original language description

In the past few decades, significant strides have been made in the domain of phytomedicines. Amongst the various medicinal plant taxa exploited for that purpose, orchids are an important representative. Ansellia africana is one such candidate taxon, which has incessantly been used in various herbal preparations for centuries. Having distributions in various parts of Africa, the plant is having potent acetyl cholinesterase inhibitory as well as antimicrobial activity. The protocorm like-bodies (PLBs) were induced by a synchronized influence of both meta-topolin (mT) and thidiazuron (TDZ) along with 1-napthalene acetic acid (NAA) and the best result was achieved at 5 μM TDZ + 15 μM mT and 5 μM NAA. Besides, the effects of six plant growth regulators (PGRs) [meta-topolin (mT), meta-topolin riboside (mTR), 6,3-methoxybenzylaminopurine (MemT) and 6,3-methoxybenzylamino-9-b-D-ribofuranosylpurine (MemTR), TDZ and picloram] on the levels of phenolic acid accumulation in the protocorm-like bodies (PLBs) biomass were assessed using the ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Coupled with that, the antioxidant potentials of the cultured biomass under various PGR treatments were also analyzed using the oxygen radical absorbance capacity (ORAC) technique. mT derivatives (mTR and MemTR) and TDZ significantly improved the synthesis and bioaccumulation of both benzoates and cinnamates in the cultured PLB biomass in comparison to control, opening a new frontier in secondary metabolite research by using orchids as source material. The highest antioxidant potential based on the oxygen radical scavenging activity (ORAC) model system was obtained in 5 μM mTR. The study strongly implies that A. africana PLBs can be manipulated on a mass scale for the production of phenolic acids exhibiting a high degree of antioxidant activity. Collectively, both PGRs and their concentrations had a comprehensive impact on induction, proliferation, biochemical accumulation of phenolic acids and the subsequent antioxidant activity of the resultant plant extracts. The present study provides important baseline information about utilization of orchid bio resources for production of prized phenolic compounds.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10401 - Organic chemistry

Project

<a href="/en/project/EF17_048%2F0007323" target="_blank" >EF17_048/0007323: Development of pre-applied research in nanotechnogy and biotechnology</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Industrial Crops and Products

ISSN

0926-6690

e-ISSN

—

Volume of the periodical

135

Issue of the periodical within the volume

SEP 1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

21-29

UT code for WoS article

000471086000003

EID of the result in the Scopus database

2-s2.0-85064383598