Accessing a Russian Wheat Aphid Resistance Gene in Bread Wheat by Long-Read Technologies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00507496" target="_blank" >RIV/61389030:_____/19:00507496 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/19:00507496
Result on the web
<a href="http://dx.doi.org/10.3835/plantgenome2018.09.0065" target="_blank" >http://dx.doi.org/10.3835/plantgenome2018.09.0065</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3835/plantgenome2018.09.0065" target="_blank" >10.3835/plantgenome2018.09.0065</a>
Alternative languages
Result language
angličtina
Original language name
Accessing a Russian Wheat Aphid Resistance Gene in Bread Wheat by Long-Read Technologies
Original language description
Russian wheat aphid (RWA) ( Kurdjumov) is a serious invasive pest of small-grain cereals and many grass species. An efficient strategy to defy aphid attacks is to identify sources of natural resistance and transfer resistance genes into susceptible crop cultivars. Revealing the genes helps understand plant defense mechanisms and engineer plants with durable resistance to the pest. To date, more than 15 RWA resistance genes have been identified in wheat ( L.) but none of them has been cloned. Previously, we genetically mapped the RWA resistance gene into an interval of 0.83 cM on the short arm of chromosome 7D and spanned it with five bacterial artificial chromosome (BAC) clones. Here, we used a targeted strategy combining traditional approaches toward gene cloning (genetic mapping and sequencing of BAC clones) with novel technologies, including optical mapping and long-read nanopore sequencing. The latter, with reads spanning the entire length of a BAC insert, enabled us to assemble the whole region, a task that was not achievable with short reads. Long-read optical mapping validated the DNA sequence in the interval and revealed a difference in the locus organization between resistant and susceptible genotypes. The complete and accurate sequence of the region facilitated the identification of new markers and precise annotation of the interval, revealing six high-confidence genes. Identification of as the most likely candidate opens an avenue for its validation through functional genomics approaches.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Genome
ISSN
1940-3372
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
180065
UT code for WoS article
000473166300003
EID of the result in the Scopus database
2-s2.0-85067985322