Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00507537" target="_blank" >RIV/61389030:_____/19:00507537 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/19:00507537 RIV/00216224:14740/19:00107607

Result on the web

<a href="http://dx.doi.org/10.1111/tpj.14304" target="_blank" >http://dx.doi.org/10.1111/tpj.14304</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/tpj.14304" target="_blank" >10.1111/tpj.14304</a>

Result language

angličtina

Original language name

Roles of RAD51 and RTEL1 in telomere and rDNA stability in Physcomitrella patens

Original language description

Telomeres and ribosomal RNA genes (rDNA) are essential for cell survival and particularly sensitive to factors affecting genome stability. Here, we examine the role of RAD51 and its antagonist, RTEL1, in the moss Physcomitrella patens. In corresponding mutants, we analyse their sensitivity to DNA damage, the maintenance of telomeres and rDNA, and repair of double-stranded breaks (DSBs) induced by genotoxins with various modes of action. While the loss of RTEL1 results in rapid telomere shortening, concurrent loss of both RAD51 genes has no effect on telomere lengths. We further demonstrate here the linked arrangement of 5S and 45S rRNA genes in P. patens. The spacer between 5S and 18S rRNA genes, especially the region downstream from the transcription start site, shows conspicuous clustering of sites with a high propensity to form quadruplex (G4) structures. Copy numbers of 5S and 18S rDNA are reduced moderately in the pprtel1 mutant, and significantly in the double pprad51-1-2 mutant, with no progression during subsequent cultivation. While reductions in 45S rDNA copy numbers observed in pprtel1 and pprad51-1-2 plants apply also to 5S rDNA, changes in transcript levels are different for 45S and 5S rRNA, indicating their independent transcription by RNA polymerase I and III, respectively. The loss of SOL (Sog One-Like), a transcription factor regulating numerous genes involved in DSB repair, increases the rate of DSB repair in dividing as well as differentiated tissue, and through deactivation of G2/M cell-cycle checkpoint allows the cell-cycle progression manifested as a phenotype resistant to bleomycin.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Journal

ISSN

0960-7412

e-ISSN

—

Volume of the periodical

98

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

16

Pages from-to

1090-1105

UT code for WoS article

000473644100011

EID of the result in the Scopus database

2-s2.0-85064500686