Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00442213" target="_blank" >RIV/68081707:_____/15:00442213 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/15:00080676
Result on the web
<a href="http://dx.doi.org/10.1111/tpj.12718" target="_blank" >http://dx.doi.org/10.1111/tpj.12718</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/tpj.12718" target="_blank" >10.1111/tpj.12718</a>
Alternative languages
Result language
angličtina
Original language name
Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants
Original language description
Arabidopsis thaliana mutants in FAS1 and FAS2 subunits of chromatin assembly factor 1 (CAF1) show progressive loss of 45S rDNA copies and telomeres. We hypothesized that homology-dependent DNA damage repair (HDR) may contribute to the loss of these repeats in fas mutants. To test this, we generated double mutants by crossing fas mutants with knock-out mutants in RAD51B, one of the Rad51 paralogs of A.thaliana. Our results show that the absence of RAD51B decreases the rate of rDNA loss, confirming the implication of RAD51B-dependent recombination in rDNA loss in the CAF1 mutants. Interestingly, this effect is not observed for telomeric repeat loss, which thus differs from that acting in rDNA loss. Involvement of DNA damage repair in rDNA dynamics in fasmutants is further supported by accumulation of double-stranded breaks (measured as -H2AX foci) in 45S rDNA. Occurrence of the foci is not specific for S-phase, and is ATM-independent. While the foci in fas mutants occur both in the tran
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Journal
ISSN
0960-7412
e-ISSN
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Volume of the periodical
81
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
198-209
UT code for WoS article
000347830900002
EID of the result in the Scopus database
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