Chemically-induced DNA de-methylation alters the effectiveness of microspore embryogenesis in triticale
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00508704" target="_blank" >RIV/61389030:_____/19:00508704 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.plantsci.2019.110189" target="_blank" >http://dx.doi.org/10.1016/j.plantsci.2019.110189</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.plantsci.2019.110189" target="_blank" >10.1016/j.plantsci.2019.110189</a>
Alternative languages
Result language
angličtina
Original language name
Chemically-induced DNA de-methylation alters the effectiveness of microspore embryogenesis in triticale
Original language description
Microspores exposed to some stress factors may display cell totipotency and could be reprogrammed towards embryogenic development. Plant breeding and genetic engineering widely use haploids/doubled haploids (DHs) derived from in vitro-cultured microspores, but the mechanism of this process remains poorly understood. Recently published data suggest that microspore embryogenesis (ME) is accompanied by changes in DNA methylation and chromatin reorganization. Here, we used two triticale DH lines (DH19 and DH28), significantly different with respect to embryogenic potential. To change DNA methylation levels, we applied two cytosine-analogs: 5-azacytidine (AC) and 2′-deoxy-5-azacytidine (DAC) treatments. We found that chemically-induced DNA demethylation caused chromatin relaxation and dysregulation of marker genes (TaTPD1-like, GSTF2, GSTA2, CHI3, Tad1, TaNF-YA7, SERK2, TaME1) related to ME. Both drugs showed significant cytotoxicity in a dose-dependent manner. We noticed that lines varied in terms of overall DNA methylation levels and responded in a different way to hypomethylation caused by the drugs. DH19 (low embryogenic) after inhibitors treatment, showed higher microspore viability, but its recalcitrancy was not overcome. For highly embryogenic DH28, we noted significantly higher effectiveness of embryo-like structure production and plant regeneration. In summary, our study provides new insight into the role of DNA methylation in ME initiation. They suggest potential benefits resulting from the utilization of epigenetic inhibitors to improve the process of DHs production.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/GA18-12197S" target="_blank" >GA18-12197S: Analysis of nuclear organization and dynamics in endosperm tissues of barley</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Science
ISSN
0168-9452
e-ISSN
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Volume of the periodical
287
Issue of the periodical within the volume
Oct
Country of publishing house
IE - IRELAND
Number of pages
12
Pages from-to
110189
UT code for WoS article
000487165500020
EID of the result in the Scopus database
2-s2.0-85069590359