Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (x Triticosecale Wittm.) after 5-azacytidine treatment
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00553335" target="_blank" >RIV/61389030:_____/21:00553335 - isvavai.cz</a>
Result on the web
<a href="http://doi.org/10.1038/s41598-021-01671-y" target="_blank" >http://doi.org/10.1038/s41598-021-01671-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-021-01671-y" target="_blank" >10.1038/s41598-021-01671-y</a>
Alternative languages
Result language
angličtina
Original language name
Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (x Triticosecale Wittm.) after 5-azacytidine treatment
Original language description
Effective microspore embryogenesis (ME) requires substantial modifications in gene expression pattern, followed by changes in the cell proteome and its metabolism. Recent studies have awakened also interest in the role of epigenetic factors in microspore de-differentiation and reprogramming. Therefore, demethylating agent (2.5-10 mu M 5-azacytidine, AC) together with low temperature (3 weeks at 4 degrees C) were used as ME-inducing tiller treatment in two doubled haploid (DH) lines of triticale and its effect was analyzed in respect of anther protein profiles, expression of selected genes (TAPETUM DETERMINANT1 (TaTPD1-like), SOMATIC EMBRYOGENESIS RECEPTOR KINASE 2 (SERK2) and GLUTATHIONE S-TRANSFERASE (GSTF2)) and ME efficiency. Tiller treatment with 5.0 mu M AC was the most effective in ME induction, it was associated with (1) suppression of intensive anabolic processes-mainly photosynthesis and light-dependent reactions, (2) transition to effective catabolism and mobilization of carbohydrate reserve to meet the high energy demand of cells during microspore reprograming and (3) effective defense against stress-inducing treatment, i.e. protection of proper folding during protein biosynthesis and effective degradation of dysfunctional or damaged proteins. Additionally, 5.0 mu M AC enhanced the expression of all genes previously identified as being associated with embryogenic potential of microspores (TaTPD1-like, SERK and GSTF2).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
11
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
18
Pages from-to
22215
UT code for WoS article
000720624200003
EID of the result in the Scopus database
2-s2.0-85119048641