TALE protein mediated overexpression of embryogenesis related marker genes in wheat microspores
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00559502" target="_blank" >RIV/60077344:_____/21:00559502 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0254629920311911?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0254629920311911?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.sajb.2020.12.004" target="_blank" >10.1016/j.sajb.2020.12.004</a>
Alternative languages
Result language
angličtina
Original language name
TALE protein mediated overexpression of embryogenesis related marker genes in wheat microspores
Original language description
Stress treatment of microspore embryogenesis leads to the reprogramming of male gametophyte development pathways, which generates haploid green plants. However, low green plant production efficiency is a major hindrance to the adoption of the double haploid (DH) production platform for wheat breeding. The aim of the current study was to induce the expression of microspore embryogenesis-associated genes by overexpressing the RKD transcription factor using Transcription activator-like effector (TALE) proteins. The TALE protein can modulate the gene expression by recognizing and binding host plant promoter sequences. Taking into consideration, we have cloned and analysed RKD promoters from wheat and triticale and used them for the custom synthesis of TaRKD-TALE protein. The isolated and purified protein was conjugated with R9 (Cys (Npys)-(D-Arg)9) cargo peptides and successfully transduced into wheat microspores. The expression of early-culture abundant (ECA1), RWP-RK domain-containing proteins (RKD1) and Tapetum determinant 1 (TPD1) marker genes in wheat linked to microspore embryogenesis were studied. The expression of all three (ECA1, TPD1 and RKD1) embryogenesis-related marker genes in the TALE transduced microspore was significantly higher than in the control. Microspore culture transduced by R9-TaRKD-TALE protein yielded a significantly higher number of embryo-like structures (ELS) and the total green plants in wheat cultivars AC Fielder. Conversely, the microspore cell death has not been significantly affected by the transduction of R9TaRKD-TALE, while showing a higher recovery at 120 h of microspore culture. This work will be helpful to exploit the TALE protein to improve our understanding of DH production and other pathways-related genes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/EF15_003%2F0000336" target="_blank" >EF15_003/0000336: Metals, plants and people</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
South African Journal of Botany
ISSN
0254-6299
e-ISSN
1727-9321
Volume of the periodical
138
Issue of the periodical within the volume
May 01
Country of publishing house
ZA - SOUTH AFRICA
Number of pages
7
Pages from-to
50-56
UT code for WoS article
000648693200004
EID of the result in the Scopus database
2-s2.0-85099249828