Bcl-2421 and Ac-DEVD-CHO Inhibit Death of Wheat Microspores
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33161328" target="_blank" >RIV/61989592:15310/16:33161328 - isvavai.cz</a>
Result on the web
<a href="http://journal.frontiersin.org/article/10.3389/fpls.2016.01931/full" target="_blank" >http://journal.frontiersin.org/article/10.3389/fpls.2016.01931/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fpls.2016.01931" target="_blank" >10.3389/fpls.2016.01931</a>
Alternative languages
Result language
angličtina
Original language name
Bcl-2421 and Ac-DEVD-CHO Inhibit Death of Wheat Microspores
Original language description
Microspore cell death and low green plant production efficiency are an integral obstacle in the development of doubled haploid production in wheat. The aim of the current study was to determine the effect of anti-apoptotic recombinant human B-cell lymphoma-2 (Bcl-2421) and caspase-3-inhibitor (Ac-DEVD-CHO) in microspore cell death in bread wheat cultivars AC Fielder and AC Andrew. Induction medium containing Bcl-2421 and Ac-DEVD-CHO yielded a significantly higher number of viable microspores, embryo-like structures and total green plants in wheat cultivars AC Fielder and AC Andrew. Total peroxidase activity was lower in Bcl-2421 treated microspore cultures at 96 h of treatment compared to control and Ac-DEVD-CHO. Electron paramagnetic resonance study of total microspore protein showed a different scavenging aktivity for Bcl-2421 and Ac-DEVD-CHO. Bcl-2421 scavenged approximately 50% hydroxyl radical (HO) formed, whereas Ac-DEVD-CHO scavenged approximately 20% of HO. Conversely, reduced caspase-3-like activities were detected in the presence of Bcl-2421 and Ac-DEVD-CHO, supporting the involvement of Bcl-2421 and Ac-DEVD-CHO in increasing microspore viability by reducing oxidative stress and caspase-3-like activity. Our results indicate that Bcl-2421 and Ac-DEVD-CHO protects cells from cell death following different pathways. Bcl-2421 prevents cell damage by detoxifying HO and suppressing caspase-3-like activity, while Ac-DEVD-CHO inhibits the cell death pathways by modulating caspase-like activity.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Plant Science
ISSN
1664-462X
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
1931
Country of publishing house
CH - SWITZERLAND
Number of pages
12
Pages from-to
"1931-1"-"1931-12"
UT code for WoS article
000390595000001
EID of the result in the Scopus database
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