Molecular mechanisms of plant steroids and study of their interaction with nuclear receptors in prostate cancer cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00524171" target="_blank" >RIV/61389030:_____/20:00524171 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/20:73600125 RIV/61989592:15310/20:73600125

Result on the web

<a href="http://doi.org/10.1016/j.fct.2020.111164" target="_blank" >http://doi.org/10.1016/j.fct.2020.111164</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fct.2020.111164" target="_blank" >10.1016/j.fct.2020.111164</a>

Result language

angličtina

Original language name

Molecular mechanisms of plant steroids and study of their interaction with nuclear receptors in prostate cancer cells

Original language description

Plant hormone brassinosteroids (BRs) have multiple important functions in plants. They have also been found to exhibit anti-tumor, anti-angiogenic and anti-proliferative activity. The experimental part of this article describes the effects of BR biosynthetic precursors on prostate cancer cells. The experiments were performed with LNCaP and DU-145 prostate cancer cell lines. These were cultivated and treated with tested BRs in different concentrations and time intervals. The tested compounds were found to affect cell viability, nuclear receptor expression, cell cycle and apoptosis in the tumor cells. IC50 concentrations were determined based on MTT test and the two most active compounds (cathasterone and 6-oxocampestanol) were used in the next experiments. Cathasterone was the most effective of all tested compounds and effectively inhibited integrity of cell spheres. It was found that both BRs had no significant effect on the cell cycle in LNCaP at IC50 concentration, while in DU-145 a significant block in G0/G1 phase after the BR treatment was observed. The effect of BRs on the nuclear steroid receptors was manifested by changes in their expression and localization. BRs demonstrated their significant effect on prostate cancer cells and the compounds have potential used in anticancer drug research and cancer treatment.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10401 - Organic chemistry

Project

<a href="/en/project/GA19-01383S" target="_blank" >GA19-01383S: Modulation of steroid receptors in human cancer cells by brassinosteroids.</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Food and Chemical Toxicology

ISSN

0278-6915

e-ISSN

—

Volume of the periodical

137

Issue of the periodical within the volume

MAR

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

111164

UT code for WoS article

000517659300024

EID of the result in the Scopus database

2-s2.0-85078850953