Overexpression of a developing xylem cDNA library in transgenic poplar generates high mutation rate specific to wood formation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00531112" target="_blank" >RIV/61389030:_____/20:00531112 - isvavai.cz</a>

Result on the web

<a href="http://doi.org/10.1111/pbi.13309" target="_blank" >http://doi.org/10.1111/pbi.13309</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/pbi.13309" target="_blank" >10.1111/pbi.13309</a>

Result language

angličtina

Original language name

Overexpression of a developing xylem cDNA library in transgenic poplar generates high mutation rate specific to wood formation

Original language description

We investigated feasibility of the Full-length complementary DNA OvereXpression (FOX) system as a mutagenesis approach in poplar, using developing xylem tissue. The main goal was to assess the overall mutation rate and if the system will increase instances of mutants affected in traits linked to the xylem tissue. Indeed, we found a high mutation rate of 17.7%, whereas 80% of all mutants were significantly affected in cellulose, lignin and/or hemicellulose. Cell wall biosynthesis is a major process occurring during xylem development. Enrichment of mutants affected in cell wall composition suggests that the tissue source for the FOX library influenced the occurrence of mutants affected in a trait linked to this tissue. Additionally, we found that FLcDNAs from mutants affected in cell wall composition were homologous to genes known to be involved in cell wall biosynthesis and most recovered FLcDNAs corresponded to genes whose native expression was highest in xylem. We characterized in detail a mutant line with increased diameter. The phenotype was caused by a poplar homolog of LONELY GUY 1 (LOG1), which encodes an enzyme in cytokinin biosynthesis and significantly increased xylem proliferation. The causative role of LOG1 in the observed phenotype was further reaffirmed by elevated cytokinin concentration in the mutant and recapitulation overexpression experiment wherein multiple independent lines phenocopied the original FOX mutant. Our experiments show that the FOX approach can be efficiently used for gene discovery and molecular interrogation of traits specific to woody perennial growth and development.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/LTAUSA17081" target="_blank" >LTAUSA17081: Hormonal mechanisms of plant acclimation to heat and cold stresses</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Biotechnology Journal

ISSN

1467-7644

e-ISSN

—

Volume of the periodical

18

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

1434-1443

UT code for WoS article

000504285300001

EID of the result in the Scopus database

2-s2.0-85077165175