The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated auxin efflux in Arabidopsis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00531204" target="_blank" >RIV/61389030:_____/20:00531204 - isvavai.cz</a>

Result on the web

<a href="http://doi.org/10.1038/s41477-020-0648-9" target="_blank" >http://doi.org/10.1038/s41477-020-0648-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41477-020-0648-9" target="_blank" >10.1038/s41477-020-0648-9</a>

Result language

angličtina

Original language name

The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated auxin efflux in Arabidopsis

Original language description

The PDK1 lipid-dependent kinase controls PIN1 and auxin transport through a phosphorylation cascade that includes AGC-type kinase D6PK. The double pdk1 mutant reveals auxin-related phenotypes such as reduced gravitropism and lateral roots. Directional intercellular transport of the phytohormone auxin mediated by PIN-FORMED (PIN) efflux carriers has essential roles in both coordinating patterning processes and integrating multiple external cues by rapidly redirecting auxin fluxes. PIN activity is therefore regulated by multiple internal and external cues, for which the underlying molecular mechanisms are not fully elucidated. Here, we demonstrate that 3 '-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE1 (PDK1), which is conserved in plants and mammals, functions as a molecular hub that perceives upstream lipid signalling and modulates downstream substrate activity through phosphorylation. Using genetic analysis, we show that the loss-of-function Arabidopsis pdk1.1 pdk1.2 mutant exhibits a plethora of abnormalities in organogenesis and growth due to defective polar auxin transport. Further cellular and biochemical analyses reveal that PDK1 phosphorylates D6 protein kinase, a well-known upstream activator of PIN proteins. We uncover a lipid-dependent phosphorylation cascade that connects membrane-composition-based cellular signalling with plant growth and patterning by regulating morphogenetic auxin fluxes.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Plants

ISSN

2055-026X

e-ISSN

—

Volume of the periodical

6

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

556-569

UT code for WoS article

000531787500006

EID of the result in the Scopus database

2-s2.0-85084451659