A fast and reliable UHPLC-MS/MS-based method for screening selected pharmacologically significant natural plant Indole alkaloids

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00531954" target="_blank" >RIV/61389030:_____/20:00531954 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/20:73604903

Result on the web

<a href="http://doi.org/10.3390/molecules25143274" target="_blank" >http://doi.org/10.3390/molecules25143274</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/molecules25143274" target="_blank" >10.3390/molecules25143274</a>

Result language

angličtina

Original language name

A fast and reliable UHPLC-MS/MS-based method for screening selected pharmacologically significant natural plant Indole alkaloids

Original language description

Many substances of secondary plant metabolism have often attracted the attention of scientists and the public because they have certain beneficial effects on human health, although the reason for their biosynthesis in the plant remains unclear. This is also the case for alkaloids. More than 200 years have passed since the discovery of the first alkaloid (morphine), and several thousand substances of this character have been isolated since then. Most often, alkaloid-rich plants are part of folk medicine with centuries-old traditions. What is particularly important to monitor for these herbal products is the spectrum and concentrations of the present active substances, which decide whether the product has a beneficial or toxic effect on human health. In this work, we present a fast, reliable, and robust method for the extraction, preconcentration, and determination of four selected alkaloids with an indole skeleton, i.e., harmine, harmaline, yohimbine, and ajmalicine, by ultra-high performance liquid chromatography coupled with tandem mass spectrometry. The applicability of the method was demonstrated for tobacco and Tribulus terrestris plant tissue, the seeds of Peganum harmala, and extract from the bark of the African tree Pausinystalia johimbe.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10609 - Biochemical research methods

Project

<a href="/en/project/EF16_019%2F0000738" target="_blank" >EF16_019/0000738: Centre for Experimental Plant Biology</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecules

ISSN

1420-3049

e-ISSN

—

Volume of the periodical

25

Issue of the periodical within the volume

14

Country of publishing house

CH - SWITZERLAND

Number of pages

15

Pages from-to

3274

UT code for WoS article

000554221500001

EID of the result in the Scopus database

2-s2.0-85088682116