In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00531959" target="_blank" >RIV/61389030:_____/20:00531959 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/20:00114308

Result on the web

<a href="http://doi.org/10.3390/plants9070868" target="_blank" >http://doi.org/10.3390/plants9070868</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/plants9070868" target="_blank" >10.3390/plants9070868</a>

Result language

angličtina

Original language name

In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes

Original language description

Rapid progress in plant molecular biology in recent years has uncovered the main players in hormonal pathways and characterized transcriptomic networks associated with hormonal response. However, the role of RNA processing, in particular alternative splicing (AS), remains largely unexplored. Here, using example genes involved in cytokinin signaling, brassinosteroid synthesis and auxin transport, we present a set of reporters devised to visualize their AS events in vivo. These reporters show a differential tissue-specific expression of certain transcripts and reveal that expression of some of the them can be changed by the application of the exogenous hormone. Finally, based on the characterized AS event of the PIN7 auxin efflux carrier, we designed a system that allows a rapid genetic screening for the factors upstream of this AS event. Our innovative toolset can be therefore highly useful for exploring novel regulatory nodes of hormonal pathways and potentially helpful for plant researchers focusing on developmental aspects of AS.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10609 - Biochemical research methods

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plants

ISSN

2223-7747

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

7

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

868

UT code for WoS article

000554288700001

EID of the result in the Scopus database

2-s2.0-85090747404