DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00538924" target="_blank" >RIV/61389030:_____/20:00538924 - isvavai.cz</a>

Result on the web

<a href="http://doi.org/10.1093/jxb/eraa370" target="_blank" >http://doi.org/10.1093/jxb/eraa370</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/eraa370" target="_blank" >10.1093/jxb/eraa370</a>

Result language

angličtina

Original language name

DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei

Original language description

Despite much recent progress, our understanding of the principles of plant genome organization and its dynamics in three-dimensional space of interphase nuclei remains surprisingly limited. Notably, it is not clear how these processes could be affected by the size of a plant's nuclear genome. In this study, DNA replication timing and interphase chromosome positioning were analyzed in seven Poaceae species that differ in their genome size. To provide a comprehensive picture, a suite of advanced, complementary methods was used: labeling of newly replicated DNA by ethynyl-2'-deoxyuridine, isolation of nuclei at particular cell cycle phases by flow cytometric sorting, three-dimensional immunofluorescence in situ hybridization, and confocal microscopy. Our results revealed conserved dynamics of DNA replication in all species, and a similar replication timing order for telomeres and centromeres, as well as for euchromatin and heterochromatin regions, irrespective of genome size. Moreover, stable chromosome positioning was observed while transitioning through different stages of interphase. These findings expand upon earlier studies in suggesting that a more complex interplay exists between genome size, organization of repetitive DNA sequences along chromosomes, and higher order chromatin structure and its maintenance in interphase, albeit controlled by currently unknown factors.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

—

Volume of the periodical

71

Issue of the periodical within the volume

20

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

6262-6272

UT code for WoS article

000605983900008

EID of the result in the Scopus database

2-s2.0-85095718378