Antibacterial, mutagenic properties and chemical characterisation of sugar bush (Protea caffra meisn.): A South African native shrub species
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00539018" target="_blank" >RIV/61389030:_____/20:00539018 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/20:73604942
Result on the web
<a href="http://doi.org/10.3390/plants9101331" target="_blank" >http://doi.org/10.3390/plants9101331</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/plants9101331" target="_blank" >10.3390/plants9101331</a>
Alternative languages
Result language
angličtina
Original language name
Antibacterial, mutagenic properties and chemical characterisation of sugar bush (Protea caffra meisn.): A South African native shrub species
Original language description
Protea caffra is used as a diarrhoeal remedy in South African herbal medicine, however, its pharmacological properties remain largely unknown. In the present study, extracts from different Protea caffra organs were screened against drug-sensitive andresistant diarrhoeagenic pathogens using the microdilution assay (minimum inhibitory concentration, MIC). Twig extracts (70% methanol, MeOH) of the plant were purified and the resultant fractions screened for antibacterial properties (MIC). The chemical profiles of the fractions were determined by Gas Chromatography- Mass Spectrometry (GC-MS), while ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to quantify the phenolic acids in the plant. The mutagenic properties of bioactive extracts were assessed using the Ames test. The extracts demonstrated weak-moderate antibacterial properties (MIC: 0.3–0.6 mg/mL). A cold ethyl acetate fraction of MeOH twig extract exhibited significant antibacterial properties (MIC = 0.078 mg/mL) against Enterococcus faecalis. The presence of antibacterial compounds (1-adamantane carboxylic acid, heptacosanol, levoglucosan, nonadecanol) in the plant was putatively confirmed based on GC-MS analysis. Furthermore, UHPLC-MS/MS analysis revealed varying concentrations of phenolic acids (0.08– 374.55 μg/g DW). Based on the Ames test, the extracts were non-mutagenic thereby suggesting their safety. To a certain degree, the current study supports the traditional use of Protea caffra to manage diarrhoea among local communities in South Africa.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/EF17_048%2F0007323" target="_blank" >EF17_048/0007323: Development of pre-applied research in nanotechnogy and biotechnology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plants
ISSN
2223-7747
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
10
Country of publishing house
CH - SWITZERLAND
Number of pages
12
Pages from-to
1-14
UT code for WoS article
000587409400001
EID of the result in the Scopus database
2-s2.0-85092339568