Mapping sites of gibberellin biosynthesis in the Arabidopsis root tip
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00542953" target="_blank" >RIV/61389030:_____/21:00542953 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/20:73604324
Result on the web
<a href="http://doi.org/10.1111/nph.16967" target="_blank" >http://doi.org/10.1111/nph.16967</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nph.16967" target="_blank" >10.1111/nph.16967</a>
Alternative languages
Result language
angličtina
Original language name
Mapping sites of gibberellin biosynthesis in the Arabidopsis root tip
Original language description
Root elongation depends on the action of the gibberellin (GA) growth hormones, which promote cell production in the root meristem and cell expansion in the elongation zone. Sites of GA biosynthesis in the roots of 7-d-old Arabidopsis thaliana seedlings were investigated using tissue-specific GA inactivation in wild-type (Col-0) or rescue of GA-deficient dwarf mutants. Tissue-specific GA depletion was achieved by ectopic expression of the GA-inactivating enzyme AtGA2ox2, which is specific for CGAs, and AtGA2ox7, which acts on CGA precursors. In addition, tissue-specific rescue of ga20ox triple and ga3ox double mutants was shown. Furthermore, GUS reporter lines for major GA20ox, GA3ox and GA2ox genes were used to observe their expression domains in the root. The effects of expressing these constructs on the lengths of the root apical meristem and cortical cells in the elongation zone confirmed that roots are autonomous for GA biosynthesis, which occurs in multiple tissues, with the endodermis a major site of synthesis. The results are consistent with the early stages of GA biosynthesis within the root occurring in the meristematic region and indicate that the penultimate step of GA biosynthesis, GA 20-oxidation, is required in both the meristem and elongation zone.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/EF16_019%2F0000738" target="_blank" >EF16_019/0000738: Centre for Experimental Plant Biology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Phytologist
ISSN
0028-646X
e-ISSN
1469-8137
Volume of the periodical
229
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
1521-1534
UT code for WoS article
000582791500001
EID of the result in the Scopus database
2-s2.0-85094103700