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Mapping sites of gibberellin biosynthesis in the Arabidopsis root tip

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73604324" target="_blank" >RIV/61989592:15310/20:73604324 - isvavai.cz</a>

  • Alternative codes found

    RIV/61389030:_____/21:00542953

  • Result on the web

    <a href="https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.16967" target="_blank" >https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.16967</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/nph.16967." target="_blank" >10.1111/nph.16967.</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Mapping sites of gibberellin biosynthesis in the Arabidopsis root tip

  • Original language description

    Root elongation depends on the action of the gibberellin (GA) growth hormones, which promote cell production in the root meristem and cell expansion in the elongation zone. Sites of GA biosynthesis in the roots of 7-d-old Arabidopsis thaliana seedlings were investigated using tissue-specific GA inactivation in wild-type (Col-0) or rescue of GA-deficient dwarf mutants. Tissue-specific GA depletion was achieved by ectopic expression of the GA-inactivating enzyme AtGA2ox2, which is specific for C19-GAs, and AtGA2ox7, which acts on C20-GA precursors. In addition, tissue-specific rescue of ga20ox triple and ga3ox double mutants was shown. Furthermore, GUS reporter lines for major GA20ox, GA3ox and GA2ox genes were used to observe their expression domains in the root. The effects of expressing these constructs on the lengths of the root apical meristem and cortical cells in the elongation zone confirmed that roots are autonomous for GA biosynthesis, which occurs in multiple tissues, with the endodermis a major site of synthesis. The results are consistent with the early stages of GA biosynthesis within the root occurring in the meristematic region and indicate that the penultimate step of GA biosynthesis, GA 20-oxidation, is required in both the meristem and elongation zone.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10611 - Plant sciences, botany

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    NEW PHYTOLOGIST

  • ISSN

    0028-646X

  • e-ISSN

  • Volume of the periodical

    229

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    14

  • Pages from-to

    1521-1534

  • UT code for WoS article

    000582791500001

  • EID of the result in the Scopus database

    2-s2.0-85094103700