Micropropagation of an ornamental shrub Disanthus cercidifolius Maxim. and assessment of genetic fidelity of regenerants using ISSR and flow cytometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00545705" target="_blank" >RIV/61389030:_____/21:00545705 - isvavai.cz</a>

Alternative codes found

RIV/00027006:_____/21:10174547 RIV/60460709:41320/21:89228 RIV/60460709:41340/21:89228

Result on the web

<a href="http://doi.org/10.1007/s11240-020-01978-0" target="_blank" >http://doi.org/10.1007/s11240-020-01978-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-020-01978-0" target="_blank" >10.1007/s11240-020-01978-0</a>

Result language

angličtina

Original language name

Micropropagation of an ornamental shrub Disanthus cercidifolius Maxim. and assessment of genetic fidelity of regenerants using ISSR and flow cytometry

Original language description

This study aimed to develop an efficient micropropagation protocol for Disanthus cercidifolius Maxim., an ornamental shrub. Sprouting buds of two genotypes (‘Trubaʼ and ‘PdSʼ) were used as an initial plant material. For the in vitro propagation experiment, the nodal segments were cultured on a MS medium supplemented with either N6-benzyladenine (BA) or zeatin (both at 0.5–3 mg l−1). As a control, MS medium without growth regulators was used. The highest number of shoots per explant (6.95 ± 0.33 in genotype ‘PdSʼ and 7.93 ± 0.41 in genotype ‘Trubaʼ) was achieved on a medium supplemented with 2 mg l−1 BA. Half-strength WPM and half-strength MS medium supplemented with indole-3-butyric acid (IBA) (0.1–1 mg l−1) were tested for in vitro rooting of the shoots. Optimal rooting performance was achieved on a half-strength WPM containing 0.5 mg l−1 IBA in genotype ‘PdSʼ, and 0.1 mg l−1 IBA in genotypes ‘Trubaʼ. The rooted plantlets were transferred ex vitro with 85% survival in genotype ‘PdSʼ and 82.5% in genotype ‘Trubaʼ. Eighteen samples from each genotype were subjected to ISSR analysis and flow cytometry to assess plant genetic fidelity after micropropagation. Fifteen ISSR primers gave rise to monomorphic patterns indicating no detected genetic variation in regenerants. Flow cytometric analysis showed that the ploidy level in all tested in vitro regenerants remained stable. The micropropagation protocol optimized here represents a reliable and efficient method for the large-scale production of plants of the ornamental shrub Disanthus cercidifolius.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell, Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

1573-5044

Volume of the periodical

144

Issue of the periodical within the volume

3

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

555-566

UT code for WoS article

000604208500003

EID of the result in the Scopus database

2-s2.0-85098723513