In vitro and ex vivo vegetative propagation and cytokinin profiles of Sceletium tortuosum (L.) N. E. Br.: a South African medicinal plant

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00549884" target="_blank" >RIV/61389030:_____/21:00549884 - isvavai.cz</a>

Result on the web

<a href="http://doi.org/10.1007/s11240-020-02001-2" target="_blank" >http://doi.org/10.1007/s11240-020-02001-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-020-02001-2" target="_blank" >10.1007/s11240-020-02001-2</a>

Result language

angličtina

Original language name

In vitro and ex vivo vegetative propagation and cytokinin profiles of Sceletium tortuosum (L.) N. E. Br.: a South African medicinal plant

Original language description

Sceletium tortuosum is a South African protected species with tremendous value in traditional and modern medicine. The plants’ mesembrine-type alkaloids are potential therapeutics for a plethora of psychological, neurological and inflammatory disorders. In our in vitro and ex vivo studies, vegetative propagation and growth of this species were investigated. Cytokinin (CK) profiles were also explored. Shoot multiplication was induced on Murashige and Skoog (MS) medium supplemented with 2.5 µM indole-3-butyric acid (IBA). In vitro-generated shoots were inoculated on MS medium supplemented with 0, 2.5, 5.0 and 10.0 µM IBA or indole-3-acetic acid (IAA). Optimal rooting (55%), mean number of roots (3.80 ± 0.83) and new leaf pairs (4.65 ± 0.67) were achieved by 10.0 µM IBA. After greenhouse acclimatization, 45–90% of plantlets survived. All ex vivo shoot cuttings rooted well (90–100%). The highest mean number of roots (11.20 ± 1.37) and root length (57.18 ± 3.85 mm) were obtained by 5.0 µM IBA. Although spontaneous rooting was observed in both experiments, auxins enhanced multiple growth parameters. Cytokinin analyses of tissue-cultured (auxin-treated) and greenhouse (untreated) plants revealed higher cytokinin levels in vitro. These investigations provide rapid and efficient propagation techniques for Sceletium tortuosum which will be valuable to conservationists and pharmaceutical companies.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell, Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

1573-5044

Volume of the periodical

145

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

191-202

UT code for WoS article

000610475700002

EID of the result in the Scopus database

2-s2.0-85099982616