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In vitro and ex vivo vegetative propagation and cytokinin profiles of Sceletium tortuosum (L.) N. E. Br.: a South African medicinal plant

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73605163" target="_blank" >RIV/61989592:15310/21:73605163 - isvavai.cz</a>

  • Result on the web

    <a href="https://link.springer.com/content/pdf/10.1007/s11240-020-02001-2.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007/s11240-020-02001-2.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s11240-020-02001-2" target="_blank" >10.1007/s11240-020-02001-2</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    In vitro and ex vivo vegetative propagation and cytokinin profiles of Sceletium tortuosum (L.) N. E. Br.: a South African medicinal plant

  • Original language description

    Sceletium tortuosum is a South African protected species with tremendous value in traditional and modern medicine. The plants’ mesembrine-type alkaloids are potential therapeutics for a plethora of psychological, neurological and inflammatory disorders. In our in vitro and ex vivo studies, vegetative propagation and growth of this species were investigated. Cytokinin (CK) profiles were also explored. Shoot multiplication was induced on Murashige and Skoog (MS) medium supplemented with 2.5 µM indole-3-butyric acid (IBA). In vitro-generated shoots were inoculated on MS medium supplemented with 0, 2.5, 5.0 and 10.0 µM IBA or indole-3-acetic acid (IAA). Optimal rooting (55%), mean number of roots (3.80 ± 0.83) and new leaf pairs (4.65 ± 0.67) were achieved by 10.0 µM IBA. After greenhouse acclimatization, 45–90% of plantlets survived. All ex vivo shoot cuttings rooted well (90–100%). The highest mean number of roots (11.20 ± 1.37) and root length (57.18 ± 3.85 mm) were obtained by 5.0 µM IBA. Although spontaneous rooting was observed in both experiments, auxins enhanced multiple growth parameters. Cytokinin analyses of tissue-cultured (auxin-treated) and greenhouse (untreated) plants revealed higher cytokinin levels in vitro. These investigations provide rapid and efficient propagation techniques for Sceletium tortuosum which will be valuable to conservationists and pharmaceutical companies.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10611 - Plant sciences, botany

Result continuities

  • Project

    <a href="/en/project/EF17_048%2F0007323" target="_blank" >EF17_048/0007323: Development of pre-applied research in nanotechnogy and biotechnology</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Plant Cell, Tissue and Organ Culture (online)

  • ISSN

    1573-5044

  • e-ISSN

  • Volume of the periodical

    145

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    12

  • Pages from-to

    191-202

  • UT code for WoS article

    000610475700002

  • EID of the result in the Scopus database

    2-s2.0-85099982616