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DIACYLGLYCEROL KINASE 5 regulates polar tip growth of tobacco pollen tubes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F22%3A00556437" target="_blank" >RIV/61389030:_____/22:00556437 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/22:10442324

  • Result on the web

    <a href="http://doi.org/10.1111/nph.17930" target="_blank" >http://doi.org/10.1111/nph.17930</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/nph.17930" target="_blank" >10.1111/nph.17930</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    DIACYLGLYCEROL KINASE 5 regulates polar tip growth of tobacco pollen tubes

  • Original language description

    Pollen tubes require a tightly regulated pectin secretion machinery to sustain the cell wall plasticity required for polar tip growth. Involved in this regulation at the apical plasma membrane are proteins and signaling molecules, including phosphoinositides and phosphatidic acid (PA). However, the contribution of diacylglycerol kinases (DGKs) is not clear. We transiently expressed tobacco DGKs in pollen tubes to identify a plasma membrane (PM)-localized isoform, and then to study its effect on pollen tube growth, pectin secretion and lipid signaling. In order to potentially downregulate DGK5 function, we overexpressed an inactive variant. Only one of eight DGKs displayed a confined localization at the apical PM. We could demonstrate its enzymatic activity and that a kinase-dead variant was inactive. Overexpression of either variant led to differential perturbations including misregulation of pectin secretion. One mode of regulation could be that DGK5-formed PA regulates phosphatidylinositol 4-phosphate 5-kinases, as overexpression of the inactive DGK5 variant not only led to a reduction of PA but also of phosphatidylinositol 4,5-bisphosphate levels and suppressed related growth phenotypes. We conclude that DGK5 is an additional player of polar tip growth that regulates pectin secretion probably in a common pathway with PI4P 5-kinases.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10601 - Cell biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    New Phytologist

  • ISSN

    0028-646X

  • e-ISSN

    1469-8137

  • Volume of the periodical

    233

  • Issue of the periodical within the volume

    5

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    18

  • Pages from-to

    2185-2202

  • UT code for WoS article

    000740760700001

  • EID of the result in the Scopus database

    2-s2.0-85122680384