DIACYLGLYCEROL KINASE 5 regulates polar tip growth of tobacco pollen tubes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F22%3A00556437" target="_blank" >RIV/61389030:_____/22:00556437 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/22:10442324
Result on the web
<a href="http://doi.org/10.1111/nph.17930" target="_blank" >http://doi.org/10.1111/nph.17930</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nph.17930" target="_blank" >10.1111/nph.17930</a>
Alternative languages
Result language
angličtina
Original language name
DIACYLGLYCEROL KINASE 5 regulates polar tip growth of tobacco pollen tubes
Original language description
Pollen tubes require a tightly regulated pectin secretion machinery to sustain the cell wall plasticity required for polar tip growth. Involved in this regulation at the apical plasma membrane are proteins and signaling molecules, including phosphoinositides and phosphatidic acid (PA). However, the contribution of diacylglycerol kinases (DGKs) is not clear. We transiently expressed tobacco DGKs in pollen tubes to identify a plasma membrane (PM)-localized isoform, and then to study its effect on pollen tube growth, pectin secretion and lipid signaling. In order to potentially downregulate DGK5 function, we overexpressed an inactive variant. Only one of eight DGKs displayed a confined localization at the apical PM. We could demonstrate its enzymatic activity and that a kinase-dead variant was inactive. Overexpression of either variant led to differential perturbations including misregulation of pectin secretion. One mode of regulation could be that DGK5-formed PA regulates phosphatidylinositol 4-phosphate 5-kinases, as overexpression of the inactive DGK5 variant not only led to a reduction of PA but also of phosphatidylinositol 4,5-bisphosphate levels and suppressed related growth phenotypes. We conclude that DGK5 is an additional player of polar tip growth that regulates pectin secretion probably in a common pathway with PI4P 5-kinases.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Phytologist
ISSN
0028-646X
e-ISSN
1469-8137
Volume of the periodical
233
Issue of the periodical within the volume
5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
18
Pages from-to
2185-2202
UT code for WoS article
000740760700001
EID of the result in the Scopus database
2-s2.0-85122680384