Fluorescence activated cell sorting—A selective tool for plant cell isolation and analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F22%3A00562417" target="_blank" >RIV/61389030:_____/22:00562417 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/22:73610133
Result on the web
<a href="https://doi.org/10.1002/cyto.a.24461" target="_blank" >https://doi.org/10.1002/cyto.a.24461</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cyto.a.24461" target="_blank" >10.1002/cyto.a.24461</a>

Result language
angličtina
Original language name
Fluorescence activated cell sorting—A selective tool for plant cell isolation and analysis
Original language description
Instrumentation for flow cytometry and sorting is designed around the assumption that samples are single-cell suspensions. However, with few exceptions, higher plants comprise complex multicellular tissues and organs, in which the individual cells are held together by shared cell walls. Single-cell suspensions can be obtained through digestion of the cells walls and release of the so-called protoplasts (plants without their cell wall). Here we describe best practices for protoplast preparation, and for analysis through flow cytometry and cell sorting. Finally, the numerous downstream applications involving sorted protoplasts are discussed.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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