Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F22%3A00567577" target="_blank" >RIV/61389030:_____/22:00567577 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/22:00129401 RIV/61989592:15310/22:73617065
Result on the web
<a href="https://doi.org/10.3389/fpls.2022.1023358" target="_blank" >https://doi.org/10.3389/fpls.2022.1023358</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fpls.2022.1023358" target="_blank" >10.3389/fpls.2022.1023358</a>

Result language
angličtina
Original language name
Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis
Original language description
The integrity of plant genetic information is constantly challenged by various internal and external factors. Therefore, plants use a sophisticated molecular network to identify, signal and repair damaged DNA. Here, we report on the identification and analysis of four uncharacterized Arabidopsis BRCT5 DOMAIN CONTAINING PROTEINs (BCPs). Proteins with the BRCT5 domain are frequently involved in the maintenance of genome stability across eukaryotes. The screening for sensitivity to induced DNA damage identified BCP1 as the most interesting candidate. We show that BCP1 loss of function mutants are hypersensitive to various types of DNA damage and accumulate an increased number of dead cells in root apical meristems upon DNA damage. Analysis of publicly available sog1 transcriptomic and SOG1 genome-wide DNA binding data revealed that BCP1 is inducible by gamma radiation and is a direct target of this key DNA damage signaling transcription factor. Importantly, bcp1 plants showed a reduced frequency of somatic homologous recombination in response to both endogenous and induced DNA damage. Altogether, we identified a novel plant-specific DNA repair factor that acts downstream of SOG1 in homology-based repair.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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