In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00575457" target="_blank" >RIV/61389030:_____/23:00575457 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.3390/horticulturae9060674" target="_blank" >https://doi.org/10.3390/horticulturae9060674</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/horticulturae9060674" target="_blank" >10.3390/horticulturae9060674</a>
Alternative languages
Result language
angličtina
Original language name
In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research
Original language description
Kohlrabi (Brassica oleracea var. gongylodes) is a vegetable cultivated for its edible stem tuber. Although valued for its nutritional properties and tolerance to abiotic stress, kohlrabi is one of the least studied brassicas. In this review, we summarize the results of our decade-long research on in vitro morphogenesis of kohlrabi, starting from 2013. Protocols for efficient in vitro regeneration with minimal requirements for external application of plant growth regulators (PGRs) have been developed, both by somatic embryogenesis (SE) and by de novo shoot organogenesis (DNSO). Efficient regeneration by direct SE was achieved using immature zygotic embryos as explants incubated on PGR-free media, and the production process was maintained in culture thanks to highly efficient secondary SE. Conversely, efficient regeneration by indirect DNSO was achieved using entire seedlings as explants and adding only cytokinin (CK) without the need for exogenous auxin. Comprehensive phytohormone analyses revealed that different exogenously applied CKs differentially affected the composition of endogenous phytohormones and induced changes in the expression of cell cycle-related genes and other genes involved in the organogenic response. The addition of high sucrose concentrations to the nutrient media failed to induce the formation of stem tubers in in vitro culture, but revealed a complex interaction with exogenously applied CKs, interfering with both the endogenous phytohormonome and the expression of organogenesis-related genes. Our work has provided substantial biotechnological advances in the field of in vitro regeneration of kohlrabi, as well as in understanding the underlying phytohormonal regulation. The review aims to introduce kohlrabi to the scientific community as a model system for both basic and applied research, while we continue seeking answers to the outstanding questions and trying to pave the way for the development of more resistant varieties.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
<a href="/en/project/EF16_019%2F0000738" target="_blank" >EF16_019/0000738: Centre for Experimental Plant Biology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
HORTICULTURAE
ISSN
2311-7524
e-ISSN
2311-7524
Volume of the periodical
9
Issue of the periodical within the volume
6
Country of publishing house
CH - SWITZERLAND
Number of pages
26
Pages from-to
674
UT code for WoS article
001017404900001
EID of the result in the Scopus database
2-s2.0-85164027441