Hormone profiling and the root proteome analysis of itpk1 mutant seedlings of barley (Hordeum vulgare) during the red-light induced photomorphogenesis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00575624" target="_blank" >RIV/61389030:_____/23:00575624 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/23:73621515

Result on the web

<a href="https://doi.org/10.1016/j.envexpbot.2023.105428" target="_blank" >https://doi.org/10.1016/j.envexpbot.2023.105428</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.envexpbot.2023.105428" target="_blank" >10.1016/j.envexpbot.2023.105428</a>

Result language

angličtina

Original language name

Hormone profiling and the root proteome analysis of itpk1 mutant seedlings of barley (Hordeum vulgare) during the red-light induced photomorphogenesis

Original language description

Inositol phosphates represent crucial primary metabolites, known as phosphate storage molecules or molecules enabling protein interactions. Inositol phosphates and their pyrophosphate forms direct the assembly of the COP9 signalosome and Cullin Ring Ligase complexes. Thus, inositol phosphates participate on protein degradation, which is a means of signal transduction for several plant hormone classes and light signaling cascade. In plants, production of inositol phosphates is maintained by several inositol phosphate kinases including the group of inositol-1,3,4-trisphosphate 5/6 kinases (ITPKs). The members of ITPKs connect plant phosphate status with light perception and hormonal balance. Photomorphogenesis in red light was investigated using allelic variants of barley Hvitpk1 mutants produced by gene editing. Barley ITPK promoter regions, conserved protein motifs, and expression profiles were investigated to characterize their evolutionary diversification and transcriptional regulation. We focused on ITPK1 relation to auxins, cytokinins, gibberellins, jasmonates, abscisic acid, and salicylic acid in red light-induced photomorphogenesis. Global proteome analyses was conducted in the mutant and wild-type (WT) seedlings grown under continuous red light. Our results demonstrate that different allelic variants of itpk1 mutants manifested defects in producing important phytohormones with consequences for root growth. Mutations in itpk1 affected hormone biosynthesis and metabolite, while proteome analysis revealed significant differences in seedling root proteomes between mutant lines. Based on our results and published data, a model of ITPK activity is proposed depicting them as regulators of hormone signal transduction at the level of protein degradation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Environmental and Experimental Botany

ISSN

0098-8472

e-ISSN

1873-7307

Volume of the periodical

213

Issue of the periodical within the volume

SEP

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

105428

UT code for WoS article

001032342500001

EID of the result in the Scopus database

2-s2.0-85163796936