N6-adenosine methylation of mRNA integrates multilevel auxin response and ground tissue development in Arabidopsis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00578511" target="_blank" >RIV/61389030:_____/23:00578511 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/23:00134766 RIV/00216208:11310/23:10471876 RIV/61989592:15310/23:73623012
Result on the web
<a href="https://doi.org/10.1242/dev.201775" target="_blank" >https://doi.org/10.1242/dev.201775</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1242/dev.201775" target="_blank" >10.1242/dev.201775</a>

Result language
angličtina
Original language name
N6-adenosine methylation of mRNA integrates multilevel auxin response and ground tissue development in Arabidopsis
Original language description
N6-methyl adenosine (m6A) is a widespread internal mRNA modification impacting the expression of numerous genes. Here, we characterize auxin-related defects among the pleiotropic phenotypes of hypomorphic Arabidopsis thaliana mutants with impaired m6A status and reveal that they show strong resistance to exogenously applied auxin. By combining major published m6A datasets, we propose that among high-confidence target transcripts emerge those encoding the main components required for auxin signaling, including the TIR1/AFB auxin receptors and ARF transcriptional regulators. We also observe subtle changes in endogenous levels of indole-3-acetic acid metabolites in these hypomorphic lines, which correlate with the methylation status of indole-3-acetic acid amidohydrolase transcripts. In addition, we reveal that reduced m6A levels lead to defects in endodermal patterning in the primary root arising from impaired timing of periclinal cell divisions. These defects can be reverted by inhibition of auxin signaling. Together, our data underline that m6A likely affects auxin-dependent processes at multiple levels.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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