EasyClick: an improved system for confocal microscopy of live roots with a user-optimized sample holder
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00582673" target="_blank" >RIV/61389030:_____/24:00582673 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26220/23:PU149505 RIV/61989592:15310/24:73625406
Result on the web
<a href="https://doi.org/10.1007/s00425-023-04293-y" target="_blank" >https://doi.org/10.1007/s00425-023-04293-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00425-023-04293-y" target="_blank" >10.1007/s00425-023-04293-y</a>
Alternative languages
Result language
angličtina
Original language name
EasyClick: an improved system for confocal microscopy of live roots with a user-optimized sample holder
Original language description
Preparation and mounting of the samples are key factors for successful live cell microscopy. To acquire biologically relevant data, it is necessary to minimize stress and avoid physical damage to plant tissues during the installation of the sample into the microscope. This is challenging, particularly when the whole plant is mounted as the living sample needs to be properly anchored in the microscopic system to obtain high-quality and high-resolution data. Here, we present a user-optimized sample holder EasyClick for live cell inverted confocal microscopic analysis of plant roots with diameters from 0.3 to 0.7 mm. The EasyClick holder was tested on an inverted confocal microscope using germinating plants of several cereals. Nevertheless, it can be directly used on other types of inverted microscopes from various producers and on different plant species. The EasyClick holder effectively restricts root lateral and vertical movements. This greatly improves the conditions for time-lapse microscopy of the samples of interest.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
<a href="/en/project/GA21-02929S" target="_blank" >GA21-02929S: Identification and characterization of imprinted genes during barley seed development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Planta
ISSN
0032-0935
e-ISSN
1432-2048
Volume of the periodical
259
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
22
UT code for WoS article
001125097100001
EID of the result in the Scopus database
2-s2.0-85179685820