Spinning disk-based superresolution microscopy for subdiffraction structured illumination imaging of living cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F19%3A00517466" target="_blank" >RIV/68378271:_____/19:00517466 - isvavai.cz</a>
Result on the web
<a href="https://jmo.fzu.cz/sites/jmo.fzu.cz/files/oldweb/2019/2019-05/jmo_19_05_obsah.pdf" target="_blank" >https://jmo.fzu.cz/sites/jmo.fzu.cz/files/oldweb/2019/2019-05/jmo_19_05_obsah.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Spinning disk-based superresolution microscopy for subdiffraction structured illumination imaging of living cells
Original language description
Superresolution microscopy enables to see previously hidden details of cellular structures. However, it requires to use high irradiation intensities that may cause artefacts and photodamage of fragile biological samples. Newly developed confocal technique and Olympus super resolution (OSR), that combines spinning disk confocal with structured illumination microscopy, represents a reliable and fast superresolution avoiding photodamage. We demonstrate an OSR microscope platform, that enables subsecond, multicolour data acquisition. It also provides access to subdiffraction structured illumination imaging. We show that OSR allows live-cell experiments without any noticeable cellular damage. OSR system has an improved lateral (∼2) and axial (∼3) resolution compared with conventional confocal imaging. Moreover, OSR is compatible with both fixed and live cell imaging.
Czech name
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Czech description
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Project
<a href="/en/project/LO1409" target="_blank" >LO1409: Center for materials analysis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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