Imaging Giardia intestinalis cellular organisation using expansion microscopy reveals atypical centrin localisation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F24%3A00599446" target="_blank" >RIV/68378050:_____/24:00599446 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/24:10485272

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0014489424001346?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0014489424001346?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.exppara.2024.108831" target="_blank" >10.1016/j.exppara.2024.108831</a>

Result language

angličtina

Original language name

Imaging Giardia intestinalis cellular organisation using expansion microscopy reveals atypical centrin localisation

Original language description

Advanced imaging of microorganisms, including protists, is challenging due to their small size. Specimen expansion prior to imaging is thus beneficial to increase resolution and cellular details. Here, we present a sample preparation workflow for improved observations of the single-celled eukaryotic pathogen Giardia intestinalis (Excavata, Metamonada). The binucleated trophozoites colonize the small intestine of humans and animals and cause a diarrhoeal disease. Their remarkable morphology includes two nuclei and a pronounced microtubular cytoskeleton enabling cell motility, attachment and proliferation. By use of expansion and confocal microscopy, we resolved in a great detail subcellular structures and organelles of the parasite cell. The acquired spatial resolution enabled novel observations of centrin localization at Giardia basal bodies. Interestingly, non-luminal centrin localization between the Giardia basal bodies was observed, which is an atypical eukaryotic arrangement. Our protocol includes antibody staining and can be used for the localization of epitope-tagged proteins, as well as for differential organelle labelling by amino reactive esters. This fast and simple technique is suitable for routine use without a superresolution microscopy equipment.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Experimental Parasitology

ISSN

0014-4894

e-ISSN

1090-2449

Volume of the periodical

266

Issue of the periodical within the volume

Nov

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

108831

UT code for WoS article

001311562900001

EID of the result in the Scopus database

2-s2.0-85203123668