Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00587315" target="_blank" >RIV/61389030:_____/24:00587315 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/24:10487293
Result on the web
<a href="https://doi.org/10.1038/s41556-024-01354-6" target="_blank" >https://doi.org/10.1038/s41556-024-01354-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41556-024-01354-6" target="_blank" >10.1038/s41556-024-01354-6</a>
Alternative languages
Result language
angličtina
Original language name
Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants
Original language description
Clathrin-mediated endocytosis is an essential cellular internalization pathway involving the dynamic assembly of clathrin and accessory proteins to form membrane-bound vesicles. The evolutionarily ancient TSET-TPLATE complex (TPC) plays an essential, but ill-defined role in endocytosis in plants. Here we show that two highly disordered TPC subunits, AtEH1 and AtEH2, function as scaffolds to drive biomolecular condensation of the complex. These condensates specifically nucleate on the plasma membrane through interactions with anionic phospholipids, and facilitate the dynamic recruitment and assembly of clathrin, as well as early- and late-stage endocytic accessory proteins. Importantly, condensation promotes ordered clathrin assemblies. TPC-driven biomolecular condensation thereby facilitates dynamic protein assemblies throughout clathrin-mediated endocytosis. Furthermore, we show that a disordered region of AtEH1 controls the material properties of endocytic condensates in vivo. Alteration of these material properties disturbs the recruitment of accessory proteins, influences endocytosis dynamics and impairs plant responsiveness. Our findings reveal how collective interactions shape endocytosis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Cell Biology
ISSN
1465-7392
e-ISSN
1476-4679
Volume of the periodical
26
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
438-449
UT code for WoS article
001161044100002
EID of the result in the Scopus database
2-s2.0-85184875928