The endocytic TPLATE complex internalizes ubiquitinated plasma membrane cargo
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F22%3A00566856" target="_blank" >RIV/61389030:_____/22:00566856 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1038/s41477-022-01280-1" target="_blank" >https://doi.org/10.1038/s41477-022-01280-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41477-022-01280-1" target="_blank" >10.1038/s41477-022-01280-1</a>
Alternative languages
Result language
angličtina
Original language name
The endocytic TPLATE complex internalizes ubiquitinated plasma membrane cargo
Original language description
Endocytosis controls the perception of stimuli by modulating protein abundance at the plasma membrane. In plants, clathrin-mediated endocytosis is the most prominent internalization pathway and relies on two multimeric adaptor complexes, the AP-2 and the TPLATE complex (TPC). Ubiquitination is a well-established modification triggering endocytosis of cargo proteins, but how this modification is recognized to initiate the endocytic event remains elusive. Here we show that TASH3, one of the large subunits of TPC, recognizes ubiquitinated cargo at the plasma membrane via its SH3 domain-containing appendage. TASH3 lacking this evolutionary specific appendage modification allows TPC formation but the plants show severely reduced endocytic densities, which correlates with reduced endocytic flux. Moreover, comparative plasma membrane proteomics identified differential accumulation of multiple ubiquitinated cargo proteins for which we confirm altered trafficking. Our findings position TPC as a key player for ubiquitinated cargo internalization, allowing future identification of target proteins under specific stress conditions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
<a href="/en/project/GM22-35680M" target="_blank" >GM22-35680M: 4D plate - Spatiotemporal dynamics of cell plate development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Plants
ISSN
2055-026X
e-ISSN
2055-0278
Volume of the periodical
8
Issue of the periodical within the volume
12
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
1467-1483
UT code for WoS article
000893871400001
EID of the result in the Scopus database
2-s2.0-85143267525