TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00575631" target="_blank" >RIV/61389030:_____/23:00575631 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.15252/embr.202254709" target="_blank" >https://doi.org/10.15252/embr.202254709</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15252/embr.202254709" target="_blank" >10.15252/embr.202254709</a>
Alternative languages
Result language
angličtina
Original language name
TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance
Original language description
Endocytosis regulates the turnover of cell surface localized receptors, which are crucial for plants to rapidly respond to stimuli. The evolutionary ancient TPLATE complex (TPC) plays an essential role in endocytosis in Arabidopsis plants. Knockout or knockdown of single TPC subunits causes male sterility and seedling lethality phenotypes, complicating analysis of the roles of TPC during plant development. Partially functional alleles of TPC subunits however only cause mild developmental deviations. Here, we took advantage of the partially functional TPLATE allele, WDXM2, to investigate a role for TPC-dependent endocytosis in receptor-mediated signaling. We discovered that reduced TPC-dependent endocytosis confers a hypersensitivity to very low doses of CLAVATA3 peptide signaling. This hypersensitivity correlated with the abundance of the CLAVATA3 receptor protein kinase CLAVATA1 at the plasma membrane. Genetic and biochemical analysis as well as live-cell imaging revealed that TPC-dependent regulation of CLAVATA3-dependent internalization of CLAVATA1 from the plasma membrane is required for shoot stem cell homeostasis. Our findings provide evidence that TPC-mediated endocytosis and degradation of CLAVATA1 is a mechanism to dampen CLAVATA3-mediated signaling during plant development.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Embo Reports
ISSN
1469-221X
e-ISSN
1469-3178
Volume of the periodical
24
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
e54709
UT code for WoS article
001029673900001
EID of the result in the Scopus database
2-s2.0-85164917625