Transgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00600386" target="_blank" >RIV/61389030:_____/24:00600386 - isvavai.cz</a>
Alternative codes found
RIV/00027006:_____/24:10177150
Result on the web
<a href="https://doi.org/10.1007/s11248-024-00388-8" target="_blank" >https://doi.org/10.1007/s11248-024-00388-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11248-024-00388-8" target="_blank" >10.1007/s11248-024-00388-8</a>
Alternative languages
Result language
angličtina
Original language name
Transgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant
Original language description
RNA silencing is an innate immune mechanism of plants against invasion by viral pathogens. Artificial microRNA (amiRNA) can be engineered to specifically induce RNA silencing against viruses in transgenic plants and has great potential for disease control. Here, we describe the development and application of amiRNA-based technology to induce resistance to soybean mosaic virus (SMV), a plant virus with a positive-sense single-stranded RNA genome. We have shown that the amiRNA targeting the SMV P1 coding region has the highest antiviral activity than those targeting other SMV genes in a transient amiRNA expression assay. We transformed the gene encoding the P1-targeting amiRNA and obtained stable transgenic Nicotiana benthamiana lines (amiR-P1-3-1-2-1 and amiR-P1-4-1-2-1). Our results have demonstrated the efficient suppression of SMV infection in the P1-targeting amiRNA transgenic plants in an expression level-dependent manner. In particular, the amiR-P1-3-1-2-1 transgenic plant showed high expression of amiR-P1 and low SMV accumulation after being challenged with SMV. Thus, a transgenic approach utilizing the amiRNA technology appears to be effective in generating resistance to SMV.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Transgenic Research
ISSN
0962-8819
e-ISSN
1573-9368
Volume of the periodical
33
Issue of the periodical within the volume
3
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
149-157
UT code for WoS article
001243252500001
EID of the result in the Scopus database
2-s2.0-85195272334