Induction of α-amylase and endosperm-imposed seed dormancy: two pioneering papers in gibberellin research

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00627737" target="_blank" >RIV/61389030:_____/25:00627737 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1007/s00425-025-04699-w" target="_blank" >https://doi.org/10.1007/s00425-025-04699-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00425-025-04699-w" target="_blank" >10.1007/s00425-025-04699-w</a>

Result language

angličtina

Original language name

Induction of α-amylase and endosperm-imposed seed dormancy: two pioneering papers in gibberellin research

Original language description

In their paper published in 1967, Russell Jones and Joseph Varner (Planta 72: 155–161) developed a bioassay based on induction of α-amylase activity in barley embryo-less half-seeds that was specific for bioactive gibberellins. The induction of α-amylase in the aleurone of barley and other cereals was to become the experimental system of choice to study gibberellin signalling. However, despite much progress in identifying the molecular events linking gibberellin action and α-amylase gene expression, in many cases their role in the process is still unclear. In 1987, Steven Groot and Cees Karssen (Planta 171:525–531) showed that germination of tomato seeds was limited by the ability of the radicle to penetrate the surrounding layers, with the endosperm forming the major barrier. They used a modified needle attached to a tensiometer to measure the force required to break through the endosperm. While in wild-type seeds, a factor from the embryo, assumed to be gibberellin, promoted breakdown of the endosperm, gibberellin-deficient seeds required an external supply of the hormone to weaken the endosperm or for it to be mechanically disrupted for germination to occur. The paradigm of seed germination being physically restricted by surrounding layers and the role of gibberellin in weakening these tissues has been confirmed in many eudicot species. Gibberellin signalling induces the production of cell-wall loosening enzymes in the micropylar endosperm adjacent to the radicle, but it is unclear whether or not this is a direct response. In both eudicot and monocot systems, there is still much to learn about the role of gibberellin signalling in germination.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Planta

ISSN

0032-0935

e-ISSN

1432-2048

Volume of the periodical

261

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

118

UT code for WoS article

001476088300001

EID of the result in the Scopus database

2-s2.0-105003821358