The transcription factor ENHANCER OF SHOOT REGENERATION 2 orchestrates cytokinin dynamics leading to developmental reprograming and green callus formation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00637493" target="_blank" >RIV/61389030:_____/25:00637493 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1093/plphys/kiaf182" target="_blank" >https://doi.org/10.1093/plphys/kiaf182</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/plphys/kiaf182" target="_blank" >10.1093/plphys/kiaf182</a>
Alternative languages
Result language
angličtina
Original language name
The transcription factor ENHANCER OF SHOOT REGENERATION 2 orchestrates cytokinin dynamics leading to developmental reprograming and green callus formation
Original language description
Callus formation and shoot regeneration are naturally triggered by stress and damage to the plant. They are also key components of tissue culture, which is crucial for gene editing, transformation, propagation, and other technologies in many species. Thus, the study of callus formation and shoot regeneration provides valuable insights into plant development. The transcription factor ENHANCER OF SHOOT REGENERATION 2/DORNROSCHEN-LIKE/BOLITA/SUPPRESSOR OF PHYTOCHROME B-4 2 (ESR2/DRNL/BOL/SOB2) promotes green callus formation in roots as well as shoot regeneration when overactive, and the phythormone cytokinin plays a prominent role in both the processes. Yet, the role of ESR2 in the cytokinin pathway has not been previously described in Arabidopsis (Arabidopsis thaliana). We found that cytokinin content and the expression of the cytokinin biosynthesis gene ISOPENTENYLTRANSFERASE 5 (IPT5) are greater in plants with high ESR2 activity. ESR2 also regulates the cytokinin signaling repressor ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 6 (AHP6), and surprisingly, ESR2-stimulated green callus formation requires IPT5 and AHP6. Therefore, ESR2 promotes both cytokinin biosynthesis and the inhibition of cytokinin signaling and, paradoxically, requires a combination of these 2 effects for green callus induction. These findings provide a foundation to better understand the processes involved in tissue reprograming toward callus formation and the role of ESR2 in callus formation and shoot regeneration.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Physiology
ISSN
0032-0889
e-ISSN
1532-2548
Volume of the periodical
198
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
kiaf182
UT code for WoS article
001494023700001
EID of the result in the Scopus database
2-s2.0-105006945840