Abutilon indicum (L.) Sweet Extracts Inhibit Key Glucose Metabolic Enzymes While Enhancing Glucose Transport in L6 Myotubes and 3T3L1 Adipocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00637641" target="_blank" >RIV/61389030:_____/25:00637641 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1155/jfbc/8252812" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1155/jfbc/8252812</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/jfbc/8252812" target="_blank" >10.1155/jfbc/8252812</a>
Alternative languages
Result language
angličtina
Original language name
Abutilon indicum (L.) Sweet Extracts Inhibit Key Glucose Metabolic Enzymes While Enhancing Glucose Transport in L6 Myotubes and 3T3L1 Adipocytes
Original language description
Background: Abutilon indicum (L.) Sweet (Malvaceae) is a traditional medicinal plant known for its antidiabetic properties in Ayurveda and other health systems. Aims: This study aims to profile metabolites in Abutilon indicum (L.) Sweet extracts (AI) and elucidate their antidiabetic mechanisms through bioinformatics and experimental methods. Study Design: The ethanolic (AIE) and aqueous (AIA) extracts were evaluated for their inhibitory effects on alpha-glucosidase and alpha-amylase, as well as their impact on glucose metabolism in 3T3-L1 adipocytes and L6 skeletal muscle cells. AIE was characterized via HPLC-DAD-QTOF-MS, with network pharmacology and molecular docking analyses used to explore molecular targets. Methods: In vitro assays were performed to assess enzyme inhibition, and cell line studies HPLC-DAD-QTOF-MS were utilized for compound characterization. Network pharmacology and molecular docking were conducted to reveal underlying antidiabetic mechanisms. Results: LC-MS-QTOF analysis identified gallic acid, stigmasterol, and riboflavin as abundant compounds. The AIE exhibited significant alpha-glucosidase (IC50 = 74.15 +/- 1.61 mu g/mL) and alpha-amylase inhibition (IC50 = 13.41 +/- 0.71 mu g/mL). Moreover, it enhanced glucose consumption in 3T3-L1 cells (IC50 = 6.25 mu g/mL) and promoted glucose uptake in L6 myotubes. Network pharmacology analyses highlighted the PI3K-Akt signaling pathway's role in facilitating glucose transport. Conclusion: The phytochemicals in AIE may contribute significantly to its antidiabetic effects, particularly through the modulation of glucose transport via the PI3K-Akt pathway. Future studies should focus on the preclinical development of safe herbal formulations utilizing these mechanisms for effective diabetes management.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF FOOD BIOCHEMISTRY
ISSN
0145-8884
e-ISSN
1745-4514
Volume of the periodical
2025
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
8252812
UT code for WoS article
001504539000001
EID of the result in the Scopus database
2-s2.0-105007787515