Temporal regulation of gene expression during auxin-triggered crown root formation in barley: an integrated approach
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00640019" target="_blank" >RIV/61389030:_____/25:00640019 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/25:73631919 RIV/61989592:15640/25:73631919
Result on the web
<a href="https://doi.org/10.1093/pcp/pcaf077" target="_blank" >https://doi.org/10.1093/pcp/pcaf077</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/pcp/pcaf077" target="_blank" >10.1093/pcp/pcaf077</a>
Alternative languages
Result language
angličtina
Original language name
Temporal regulation of gene expression during auxin-triggered crown root formation in barley: an integrated approach
Original language description
Cereal plants possess a fibrous root system in which crown roots form the major component. Crown roots develop post-embryonically from the lower, mostly underground nodes of the stem base. A strict spatiotemporal regulation of gene expression governs this process. Much of the knowledge about signaling pathways controlling crown root initiation (CRI) and development comes from rice. However, distinct regulatory mechanisms may have evolved in other cereals to adapt to different habitats.In this study, using a Crown Root Inducible System (CRIS), we aimed to investigate the early molecular regulation of barley CRI. We revealed dynamic transcriptomic changes within the first 24 hours following auxin stimulation. Among the differentially expressed genes, we identified orthologs of important CRI regulators from other cereals, demonstrating that CRIS is suitable for uncovering genes involved in CRI. Further, ATAC-seq revealed that CRI relies on changes in chromatin accessibility near root development-related genes and within distal intergenic regions.Finally, we focused on two transcription factors, HvNAC013 and CBF12C, which likely play roles in both CRI and abiotic stresses. By performing DAP-seq, we determined their genome-wide binding sites and identified their potential downstream targets. Data suggest that CBF12C is a putative target of HvNAC013, along with other auxin-responsive genes implicated in CRI. We propose that HvNAC013 and CBF12C function as part of a transcription factor network involved in CRI and potentially modulate root architecture in response to environmental conditions. This study enhances our understanding of the CRI mechanism in barley.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant and Cell Physiology
ISSN
0032-0781
e-ISSN
1471-9053
Volume of the periodical
66
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
1284-1303
UT code for WoS article
001555698800001
EID of the result in the Scopus database
2-s2.0-105017055503