Transcriptional changes during crown-root development and emergence in barley (Hordeum vulgare L.)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F24%3A73625094" target="_blank" >RIV/61989592:15640/24:73625094 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/24:73625094 RIV/00209805:_____/24:00079824

Result on the web

<a href="https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-024-05160-y" target="_blank" >https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-024-05160-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12870-024-05160-y" target="_blank" >10.1186/s12870-024-05160-y</a>

Result language

angličtina

Original language name

Transcriptional changes during crown-root development and emergence in barley (Hordeum vulgare L.)

Original language description

Background Roots play an important role during plant growth and development, ensuring water and nutrient uptake. Understanding the mechanisms regulating their initiation and development opens doors towards root system architecture engineering. Results Here, we investigated by RNA-seq analysis the changes in gene expression in the barley stem base of 1 day-after-germination (DAG) and 10DAG seedlings when crown roots are formed. We identified 2,333 genes whose expression was lower in the stem base of 10DAG seedlings compared to 1DAG seedlings. Those genes were mostly related to basal cellular activity such as cell cycle organization, protein biosynthesis, chromatin organization, cytoskeleton organization or nucleotide metabolism. In opposite, 2,932 genes showed up-regulation in the stem base of 10DAG seedlings compared to 1DAG seedlings, and their function was related to phytohormone action, solute transport, redox homeostasis, protein modification, secondary metabolism. Our results highlighted genes that are likely involved in the different steps of crown root formation from initiation to primordia differentiation and emergence, and revealed the activation of different hormonal pathways during this process. Conclusions This whole transcriptomic study is the first study aiming at understanding the molecular mechanisms controlling crown root development in barley. The results shed light on crown root emergence that is likely associated with a strong cell wall modification, death of the cells covering the crown root primordium, and the production of defense molecules that might prevent pathogen infection at the site of root emergence.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC PLANT BIOLOGY

ISSN

1471-2229

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

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UT code for WoS article

001229472600003

EID of the result in the Scopus database

2-s2.0-85193935057