TRB proteins in moss reveal their evolutionarily conserved roles in plant development and telomere maintenance
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00641858" target="_blank" >RIV/61389030:_____/25:00641858 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/25:00641858 RIV/67985939:_____/25:00641858 RIV/00216224:14310/25:00142986
Result on the web
<a href="https://doi.org/10.1111/tpj.70574" target="_blank" >https://doi.org/10.1111/tpj.70574</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/tpj.70574" target="_blank" >10.1111/tpj.70574</a>
Alternative languages
Result language
angličtina
Original language name
TRB proteins in moss reveal their evolutionarily conserved roles in plant development and telomere maintenance
Original language description
Telomere repeat binding (TRB) proteins are plant-specific proteins with a unique domain structure distinct from telomerebinding proteins in animals and yeast. While extensively studied in seed plants, their role in early-diverging plant lineages remains largely unexplored. Here, we investigate TRB proteins in a model moss, Physcomitrium patens, to assess their evolutionary conservation and functional significance. Functional analysis using single knockout mutants revealed that individual PpTRB genes are essential for normal development, with mutants exhibiting defects in the two-dimensional (protonemal) stage, and more prominently, in the formation of three-dimensional (gametophore) structures. Some double mutants displayed telomere shortening, a phenotype also observed in TRB-deficient seed plants, indicating a conserved role for TRBs in telomere maintenance. Transcriptome profiling of TRB mutants revealed altered expression of genes associated with transcriptional regulation and stimulus response in protonema. Subcellular localization studies across various plant cell types confirmed that PpTRBs, like their seed plant counterparts, localize prevalently to the plant nucleus and mutually interact. In bryophytes, TRBs form a monophyletic group that mirrors the species phylogeny, whereas in seed plants, TRBs have diversified into two distinct monophyletic groups. Our findings provide the first comprehensive characterization of TRB proteins in non-vascular plants and demonstrate their conserved roles in telomere maintenance, with additional implications for plant development and gene regulation across land plant lineages.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Journal
ISSN
0960-7412
e-ISSN
1365-313X
Volume of the periodical
124
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
21
Pages from-to
e70574
UT code for WoS article
001614719100001
EID of the result in the Scopus database
2-s2.0-105021767368