Illuminating the subcellular maze: fluorescence-activated organelle sorting in plant sciences

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F26%3A00645825" target="_blank" >RIV/61389030:_____/26:00645825 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1093/jxb/eraf490" target="_blank" >https://doi.org/10.1093/jxb/eraf490</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/eraf490" target="_blank" >10.1093/jxb/eraf490</a>

Result language

angličtina

Original language name

Illuminating the subcellular maze: fluorescence-activated organelle sorting in plant sciences

Original language description

The isolation of organelles is critical for gaining a deeper understanding of their functions in intracellular processes, not only at the cellular but also at the multicellular, organ, and organism levels. Isolating them into pure fractions allows for the reduction of sample complexity, thereby ensuring high quality downstream analysis, such as in protein localization studies. Since the mid-20th century, new methods of subcellular fractionation have constantly emerged. Conventional fractionation approaches based on (ultra)centrifugation typically focus on isolating only one type of organelle. Moreover, their resolving power may be inadequate for improving the limit of detection of downstream applications. Fluorescence-activated organelle sorting (FAOS) is a versatile and advanced technique that is gaining popularity due to its high efficiency. This efficiency refers to the ability to monitor organelle isolation live and to sort multiple organelle populations simultaneously from a single sample. This review offers an overview of the usage of FAOS and highlights its promising prospects within the realm of plant sciences. FAOS shows great potential for applications in both the functional and structural analysis of plant organelles while serving as a valuable isolation tool for downstream applications, including 'omics' studies.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2026

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

1460-2431

Volume of the periodical

77

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

120-133

UT code for WoS article

001625144800001

EID of the result in the Scopus database

2-s2.0-105025057916