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Evaluation and comparison of therapeutic monitoring of whole-blood levels of cyclosporin A and its metabolites in renal transplantation by HPLC and RIA methods

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F01%3AA0900ANI" target="_blank" >RIV/61988987:17110/01:A0900ANI - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Evaluation and comparison of therapeutic monitoring of whole-blood levels of cyclosporin A and its metabolites in renal transplantation by HPLC and RIA methods

  • Original language description

    Background: The aim of the work was to evaluate the possibility to estimate the level of cyclosporin A (CyA) metabolites as the difference of radioimmunoassay (RIA) non-specific and RIA specific methods. Methods: Blood samples of renal transplant patients were analyzed by three different methods: RIA specific method (CYCLO-Trac, DiaSorin, USA) (RIA(SP)), RIA non-specific method (Immunotech, Czech Republic) (RIA(NS)), and high performance liquid chromatography (HPLC) method. Conclusion: The combination of both the specific and non-specific methods for the determination of CyA presents an improved means for the TDM of CyA and CyA metabolites in renal transplant patients. Moreover, a combination of both methods can help to elucidate some unexpected events, such as the persistence of high cyclosporin blood levels. (C) 2001 Elsevier Science B.V. All rights reserved.

  • Czech name

    Srovnání terapeutického monitorování cyklosporinu A a jeho metabolitů u pacientů s transplantovanými ledvinami při použití HPLC a RIA metod

  • Czech description

    Cílem práce bylo zjistit možnost vlivu metabolitů cyklosporinu A na rozdíl výsledků specifické a nespecifické RIA metody. Krevní vzorky pacientů s transplantovanými metodami byly analyzovány 3 různými metodami: (CYCLO-Trac, DiaSorin, USA) (RIA(SP)), RIAnespecifická and RIA specifická methods a high performance liquid chromatography (HPLC) metoda. Kombinace spelcifické a nespecifické RIA metody při stanovení cyklosporinu A zlepšuje možnosti TDM cyklosporinu a může vysvětlit i některé neočekávané vysokéhladiny cyklosporinu A.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FR - Pharmacology and apothecary chemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    R - Projekt Ramcoveho programu EK

Others

  • Publication year

    2001

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Clin. Chim. Acta

  • ISSN

    0009-8981

  • e-ISSN

  • Volume of the periodical

    310

  • Issue of the periodical within the volume

    20

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    6

  • Pages from-to

  • UT code for WoS article

    000170811100008

  • EID of the result in the Scopus database