IDH Mutation Analysis in Glioma Patients by CADMA Compared with SNaPshot Assay and two Immunohistochemical Methods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F18%3AA1901TQT" target="_blank" >RIV/61988987:17110/18:A1901TQT - isvavai.cz</a>

Alternative codes found

RIV/00843989:_____/19:E0107866 RIV/61989592:15110/19:73588666

Result on the web

<a href="http://dx.doi.org/10.1007/s12253-018-0413-9" target="_blank" >http://dx.doi.org/10.1007/s12253-018-0413-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12253-018-0413-9" target="_blank" >10.1007/s12253-018-0413-9</a>

Result language

angličtina

Original language name

IDH Mutation Analysis in Glioma Patients by CADMA Compared with SNaPshot Assay and two Immunohistochemical Methods

Original language description

Mutations in IDH1/2 genes are a marker of good prognosis for glioma patients, associated with low grade gliomas and secondaryglioblastomas. Immunohistochemistry and Sanger sequencing are current standards for IDH1/2 genotyping while many othermethods exist. The aim of this study was to validate Competitive amplification of differentially melting amplicons (CADMA)PCR for IDH genotyping by comparison with SNaPshot assay and two immunohistochemical methods. In our study, 87 gliomapatients (46 from Olomouc and 41 from Ostrava) were analyzed. IDH1/2 mutations in native bioptical samples were analyzed atDNA level by CADMA and SNaPshot while IDH1 mutations in FFPE samples were analyzed at protein level by two IHCmethods. CADMA PCR sensitivity for IDH1 was 96.4% and specificity 100% for 86 concluded samples. SNaPshot assaysensitivity was 92.9% and specificity of 100% for 85 concluded samples. IHC in the laboratory no. 2 reached sensitivity85.7% and specificity 100% for 86 concluded samples. IHC in the laboratory no. 4 reached sensitivity of 96.4% and specificityof 79.7% in 74 concluded samples. Only one IDH2 mutation was found by SNaPshot while CADMA yielded false negativeresult. In conclusion, CADMA is a valid method for IDH1 p.(R132H) testing with higher sensitivity than SNaPshot assay. Also,molecular genetic methods of IDH1 testing from native samples were more robust than IHC from FFPE.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

30102 - Immunology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Pathology & Oncology Research

ISSN

1219-4956

e-ISSN

1532-2807

Volume of the periodical

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Issue of the periodical within the volume

3/2018

Country of publishing house

HU - HUNGARY

Number of pages

8

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

2-s2.0-85044189432