Change of activity and amount of Rubisco enzyme in European beech under elevated CO2 concentration

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F07%3AA0800KR6" target="_blank" >RIV/61988987:17310/07:A0800KR6 - isvavai.cz</a>

Alternative codes found

RIV/61988987:17310/07:A1000KR6

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Změna aktivity a množství enzymu Rubisco v sazenicích buku lesního pod vlivem zvýšené koncentrace CO2

Original language description

In this study, we estimated the major photosynthetic responses of European beech (Fagus sylvatica L.) seedlings to the impact of elevated carbon dioxide concentration ([CO2]). We compared diurnal changes in Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) enzyme activity observed in vivo and in vitro and also amount of this enzyme. Rubisco catalyses carboxylation of ribulose-1,5-bisphosphate (RuBP), the first step of photosynthetic carbon assimilation, in competition with oxygenation of RuBP that leads to the photorespiratory pathway. Our analysis shows that the short-term exposure to elevated [CO2] doesnot change the maximum carboxylation rate of Rubisco in vivo. In contrary, we found lower total activity in vitro; however, the amount of Rubisco was higher in trees cultivated under elevated as compared to ambient [CO2]. Treatment in elevated [CO2] thus led to the decrease of total Rubisco activity per unit amount pointing to that higher portion of Rubisco remains inactive.

Czech name

Změna aktivity a množství enzymu Rubisco v sazenicích buku lesního pod vlivem zvýšené koncentrace CO2

Czech description

In this study, we estimated the major photosynthetic responses of European beech (Fagus sylvatica L.) seedlings to the impact of elevated carbon dioxide concentration ([CO2]). We compared diurnal changes in Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) enzyme activity observed in vivo and in vitro and also amount of this enzyme. Rubisco catalyses carboxylation of ribulose-1,5-bisphosphate (RuBP), the first step of photosynthetic carbon assimilation, in competition with oxygenation of RuBP that leads to the photorespiratory pathway. Our analysis shows that the short-term exposure to elevated [CO2] doesnot change the maximum carboxylation rate of Rubisco in vivo. In contrary, we found lower total activity in vitro; however, the amount of Rubisco was higher in trees cultivated under elevated as compared to ambient [CO2]. Treatment in elevated [CO2] thus led to the decrease of total Rubisco activity per unit amount pointing to that higher portion of Rubisco remains inactive.

Type

D - Article in proceedings

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/GA522%2F06%2F0930" target="_blank" >GA522/06/0930: Changes in Rubisco amount and activity under the impact of elevated CO2 concentration</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Beskydy

ISBN

80-7157-966-1

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

221-226

Publisher name

MZLU

Place of publication

Brno, Česká republika

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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