Identification of Pelomyxa palustris Endosymbionts
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F17%3AA1801OCW" target="_blank" >RIV/61988987:17310/17:A1801OCW - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.protis.2017.06.001" target="_blank" >http://dx.doi.org/10.1016/j.protis.2017.06.001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.protis.2017.06.001" target="_blank" >10.1016/j.protis.2017.06.001</a>
Alternative languages
Result language
angličtina
Original language name
Identification of Pelomyxa palustris Endosymbionts
Original language description
Pelomyxa palustris is a giant anaerobic/microaerobic amoeba, characterized by a number of exceptional cytological and physiological features, among them the presumed absence of energy producing organelles and the presence of endosymbiotic bacteria. These endosymbionts have been previously distinguished as: a large rectangular-shaped Gram-variable rod with a central cleft; a slender Gram-negative rod; and a slender Gram-positive rod. Using DNA extracted from P. palustris cysts, we have obtained three SSU rRNA gene sequences. We have determined that these sequences are affiliated to three different prokaryotic genera: Methanosaeta (a methanogenic archaea), Syntrophorhabdus (a syntrophic Gram-negative bacteria) and Rhodococcus (an aerobic chemoorganotrophic Gram-positive bacteria). To our knowledge, it is the first time that Syntrophorhabdus has been described as an endosymbiont in association with a methanogen. Strikingly, no traces of Methanobacterium formicicum could be detected, despite this methanogen had allegedly been isolated from trophozoites of P. palustris. It seems that the host and the endosymbionts have established a multipartite syntrophic consortium resembling to some extent those found in sewage treatment plants. (C) 2017 Elsevier GmbH. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PROTIST
ISSN
1434-4610
e-ISSN
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Volume of the periodical
168
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
17
Pages from-to
408-424
UT code for WoS article
000410031300003
EID of the result in the Scopus database
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