Effects of selenate and red Se-nanoparticles on the photosynthetic apparatus of Nicotiana tabacum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F19%3AA2001W3A" target="_blank" >RIV/61988987:17310/19:A2001W3A - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s11120-018-0599-4" target="_blank" >https://link.springer.com/article/10.1007/s11120-018-0599-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11120-018-0599-4" target="_blank" >10.1007/s11120-018-0599-4</a>
Alternative languages
Result language
angličtina
Original language name
Effects of selenate and red Se-nanoparticles on the photosynthetic apparatus of Nicotiana tabacum
Original language description
Selenium (Se) is a natural trace element, which shifts its action in a relatively narrow concentration range from nutritional role to toxicity. Although it has been well established that in plants chloroplasts are among the primary targets, the mechanism of toxicity on photosynthesis is not well understood. Here, we compared selenate and red-allotrope elemental selenium nanoparticles (red nanoSe) in in vitro tobacco cultures to investigate their effects on the structure and functions of the photosynthetic machinery. Selenate at 10mg/L concentration retarded plant growth; it also led to a decreased chlorophyll content, accompanied with an increase in the carotenoid-to-chlorophyll ratio. Structural examinations of the photosynthetic machinery, using electron microscopy, small-angle neutron scattering and circular dichroism spectroscopy, revealed significant perturbation in the macro-organization of the pigment-protein complexes and sizeable shrinkage in the repeat distance of granum thylakoid membranes. As shown by chlorophyll a fluorescence transient measurements, these changes in the ultrastructure were associated with a significantly diminished photosystem II activity and a reduced performance of the photosynthetic electron transport, and an enhanced capability of non-photochemical quenching. These changes in the structure and function of the photosynthetic apparatus explain, at least in part, the retarded growth of plantlets in the presence of 10mg/L selenate. In contrast, red nanoSe, even at 100mg/L and selenate at 1mg/L, exerted no negative effect on the growth of plantlets and affected only marginally the thylakoid membrane ultrastructure and the photosynthetic functions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Photosynthesis Research
ISSN
0166-8595
e-ISSN
1573-5079
Volume of the periodical
139
Issue of the periodical within the volume
1-3
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
12
Pages from-to
449-460
UT code for WoS article
000458553100034
EID of the result in the Scopus database
2-s2.0-85055987806