Salt stress induces paramylon accumulation and fine-tuning the macro-organization of thylakoid membranes in Euglena gracilis cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F21%3AA2202AX0" target="_blank" >RIV/61988987:17310/21:A2202AX0 - isvavai.cz</a>
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fpls.2021.725699/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fpls.2021.725699/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fpls.2021.725699" target="_blank" >10.3389/fpls.2021.725699</a>
Alternative languages
Result language
angličtina
Original language name
Salt stress induces paramylon accumulation and fine-tuning the macro-organization of thylakoid membranes in Euglena gracilis cells
Original language description
The effect of salt stress condition on the growth, morphology, photosynthetic performance and paramylon content were examined in the mixotrophic, unicellular, flagellate Euglena gracilis. We found that salt stress negatively influenced the cell growth, accompanied by a decrease of the chlorophyll content. Circular dichroism spectroscopy revealed changes in the macro-organization of pigment-protein complexes due to salt treatment, while small-angle neutron scattering (SANS) investigations suggested reduction in the thylakoid stacking, an effect confirmed by transmission electron microscopy. At the same time, analysis of the thylakoid membrane complexes using native-PAGE revealed no significant change in the composition of supercomplexes of the photosynthetic apparatus. Salt stress did not substantially affect the photosynthetic activity, as reflected by the facts that chlorophyll fluorescence yield, electron transport rate and energy transfer between the photosystems did not change considerably in the salt-grown cells. We have observed notable increases in the carotenoid-to-chlorophyll ratio and the accumulation of paramylon in the salt-treated cells. We propose that accumulation of storage polysaccharides and changes in the pigment composition and thylakoid membrane organization help the adaptation of Euglena gracilis cells to salt stress and contribute to the maintenance of cellular processes under stress conditions.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/GA19-13637S" target="_blank" >GA19-13637S: Roles of non-bilayer lipids and non-lamellar lipid phases in the structure, dynamics and function of plant thylakoid membranes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Plant Science
ISSN
1664-462X
e-ISSN
1664-462X
Volume of the periodical
12
Issue of the periodical within the volume
NOV 16 2021
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
1-15
UT code for WoS article
000725707400001
EID of the result in the Scopus database
2-s2.0-85120446495