Redox transients of P680 associated with the incremental chlorophyll-a fluorescence yield rises elicited by a series of saturating flashes in diuron-treated Photosystem II core complex of Thermosynechococcus vulcanus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F19%3AA2001YBO" target="_blank" >RIV/61988987:17310/19:A2001YBO - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/ppl.12945" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/ppl.12945</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/ppl.12945" target="_blank" >10.1111/ppl.12945</a>
Alternative languages
Result language
angličtina
Original language name
Redox transients of P680 associated with the incremental chlorophyll-a fluorescence yield rises elicited by a series of saturating flashes in diuron-treated Photosystem II core complex of Thermosynechococcus vulcanus
Original language description
Recent chlorophyll-a fluorescence yield measurements, using single-turnover saturating flashes (STSFs), have revealed the involvement of a rate-limiting step in the reactions following the charge separation induced by the first flash. As also shown here, in diuron-inhibited PSII core complexes isolated from Thermosynechococcus vulcanus the fluorescence maximum could only be reached by a train of STSFs. In order to elucidate the origin of the fluorescence yield increments in STSF series, we performed transient absorption measurements at 819nm, reflecting the photooxidation and re-reduction kinetics of the primary electron donor P680. Upon single flash excitation of the dark-adapted sample, the decay kinetics could be described with lifetimes of 17ns (approximate to 50%) and 167ns (approximate to 30%), and a longer-lived component (approximate to 20%). This kinetics are attributed to re-reduction of P680(center dot+) by the donor side of PSII. In contrast, upon second-flash (with t between 5s and 100ms) or repetitive excitation, the 819nm absorption changes decayed with lifetimes of about 2ns (approximate to 60%) and 10ns (approximate to 30%), attributed to recombination of the primary radical pair P680(center dot+)Pheo(center dot-), and a small longer-lived component (approximate to 10%). These data confirm that only the first STSF is capable of generating stable charge separation-leading to the reduction of Q(A); and thus, the fluorescence yield increments elicited by the consecutive flashes must have a different physical origin. Our double-flash experiments indicate that the rate-limiting steps, detected by chlorophyll-a fluorescence, are not correlated with the turnover of P680.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiologia Plantarum
ISSN
0031-9317
e-ISSN
1399-3054
Volume of the periodical
166
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
22-32
UT code for WoS article
000466108300004
EID of the result in the Scopus database
2-s2.0-85064712279