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Tetracycline Induces the Formation of Biofilm of Bacteria from Different Phases of Wastewater Treatment

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F20%3AA210261L" target="_blank" >RIV/61988987:17310/20:A210261L - isvavai.cz</a>

  • Alternative codes found

    RIV/61388971:_____/20:00534588 RIV/00216208:11310/20:10422140 RIV/61989100:27710/20:10245611

  • Result on the web

    <a href="https://www.mdpi.com/2227-9717/8/8/989" target="_blank" >https://www.mdpi.com/2227-9717/8/8/989</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3390/pr8080989" target="_blank" >10.3390/pr8080989</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Tetracycline Induces the Formation of Biofilm of Bacteria from Different Phases of Wastewater Treatment

  • Original language description

    The study monitored the effect of tetracycline on bacterial biofilm formation and compared biofilm formation by resistant bacterial strains in different phases of the wastewater treatment process in wastewater treatment plant (WWTP). The crystal violet staining method was used to evaluate the biofilm formation. Biofilm-related bacterial properties were characterized by hydrophobicity, autoaggregation and motility tests. The relative abundance of tetracycline resistance genes (tetW, tetM, tetO, tetA and tetB) in wastewaters were subsequently quantified using qPCR. The results show that the isolates from the nitrification tank produce biofilm with up to 10 times greater intensity relative to the isolates from the sedimentation tank. In isolates of Aeromonas sp. from the nitrification tank, increased biofilm production in the occurrence of tetracycline from a concentration of 0.03125 µg/mL was observed. The tetW gene showed the highest relative abundance out of all the tested genes. From the sampling points, its abundance was the highest in the sedimentation tank of the WWTP. Based on these results, it can be assumed that resistant bacteria are able to form a biofilm and sub-inhibitory tetracycline concentrations induce biofilm formation. WWTPs thus represent a reservoir of antibiotic resistance genes and contribute to the spread of resistance in the natural environment.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    <a href="/en/project/EF16_019%2F0000853" target="_blank" >EF16_019/0000853: Institute of Environmental Technology - Excellent Research</a><br>

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Processes

  • ISSN

    2227-9717

  • e-ISSN

    2227-9717

  • Volume of the periodical

    8

  • Issue of the periodical within the volume

    8

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    20

  • Pages from-to

    1-20

  • UT code for WoS article

    000567672100001

  • EID of the result in the Scopus database

    2-s2.0-85090026580